Plasmid Midiprep Kits

The kits provide an easy and reliable method to purify plasmid DNA from 50-100 mL bacterial cultures in less than 60 minutes. The DNA is ready for downstream applications such as transfection, RFLP, DNA amplification, and automated sequencing. 

  • Midiprep kit: 100-300 µg of high copy plasmid DNA can be isolated from 50 mL culture.
  • Midiprep-II kit: 200-500 µg of high copy plasmid DNA can be isolated from 100 mL culture.
  • Endofree Midiprep kit:100-300 µg DNA yield. Purified DNA can be used for transfection in endotoxin sensitive cells or microinjection. Endotoxin < 0.1 EU/µg DNA.
  • Endofree Midiprep-II kit: 200-500 µg DNA yield. Purified DNA can be used for transfection in endotoxin sensitive cells or microinjection. Endotoxin < 0.1 EU/µg DNA.
  • ezFilter is used to clear cell lysate instead of centrifugation.
18 products

  • Plasmid Midiprep Kit (10 Preps) Plasmid Midiprep Kit (10 Preps)

    Plasmid Midiprep Kit (10 Preps)

    EasyPrep™ Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 100-250 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrepTM Plasmid Midiprep Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Midiprep Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD03-01PD03-11 PD03-02PD03-12 PD03-03PD03-13 Midiprep DNA Columns 10 25 50 EasyFilter 10(PD03-11) 25(PD03-12) 50(PD03-13) Buffer A1 30 ml 80 ml 160 ml Buffer A2 30 ml 80 ml 160 ml Buffer A3 45 ml 100 ml 250 ml Elution Buffer 10 ml 20 ml 40 ml Column Buffer 15 ml 30 ml 60 ml RNase A 110 µl 275 µl 540 µl 15ml conical tubes 10 25 25 Manual 1 1 1  

    $56.00

  • Plasmid Midiprep Kit (25 Preps) Plasmid Midiprep Kit (25 Preps)

    Plasmid Midiprep Kit (25 Preps)

    EasyPrep™ Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 100-250 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrepTM Plasmid Midiprep Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Midiprep Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD03-01PD03-11 PD03-02PD03-12 PD03-03PD03-13 Midiprep DNA Columns 10 25 50 EasyFilter 10(PD03-11) 25(PD03-12) 50(PD03-13) Buffer A1 30 ml 80 ml 160 ml Buffer A2 30 ml 80 ml 160 ml Buffer A3 45 ml 100 ml 250 ml Elution Buffer 10 ml 20 ml 40 ml Column Buffer 15 ml 30 ml 60 ml RNase A 110 µl 275 µl 540 µl 15ml conical tubes 10 25 25 Manual 1 1 1  

    $135.00

  • Plasmid Midiprep Kit (50 Preps)

    Plasmid Midiprep Kit (50 Preps)

    EasyPrep™ Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 100-250 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrepTM Plasmid Midiprep Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Midiprep Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD03-01PD03-11 PD03-02PD03-12 PD03-03PD03-13 Midiprep DNA Columns 10 25 50 EasyFilter 10(PD03-11) 25(PD03-12) 50(PD03-13) Buffer A1 30 ml 80 ml 160 ml Buffer A2 30 ml 80 ml 160 ml Buffer A3 45 ml 100 ml 250 ml Elution Buffer 10 ml 20 ml 40 ml Column Buffer 15 ml 30 ml 60 ml RNase A 110 µl 275 µl 540 µl 15ml conical tubes 10 25 25 Manual 1 1 1  

    $225.00

  • EasyFilter Plasmid Midiprep Kit (10 Preps) EasyFilter Plasmid Midiprep Kit (10 Preps)

    EasyFilter Plasmid Midiprep Kit (10 Preps)

    EasyPrep™ Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 100-250 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrepTM Plasmid Midiprep Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Midiprep Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD03-01PD03-11 PD03-02PD03-12 PD03-03PD03-13 Midiprep DNA Columns 10 25 50 EasyFilter 10(PD03-11) 25(PD03-12) 50(PD03-13) Buffer A1 30 ml 80 ml 160 ml Buffer A2 30 ml 80 ml 160 ml Buffer A3 45 ml 100 ml 250 ml Elution Buffer 10 ml 20 ml 40 ml Column Buffer 15 ml 30 ml 60 ml RNase A 110 µl 275 µl 540 µl 15ml conical tubes 10 25 25 Manual 1 1 1  

    $78.00

  • EasyFilter Plasmid Midiprep Kit (25 Preps)

    EasyFilter Plasmid Midiprep Kit (25 Preps)

    EasyPrep™ Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 100-250 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrepTM Plasmid Midiprep Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Midiprep Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD03-01PD03-11 PD03-02PD03-12 PD03-03PD03-13 Midiprep DNA Columns 10 25 50 EasyFilter 10(PD03-11) 25(PD03-12) 50(PD03-13) Buffer A1 30 ml 80 ml 160 ml Buffer A2 30 ml 80 ml 160 ml Buffer A3 45 ml 100 ml 250 ml Elution Buffer 10 ml 20 ml 40 ml Column Buffer 15 ml 30 ml 60 ml RNase A 110 µl 275 µl 540 µl 15ml conical tubes 10 25 25 Manual 1 1 1  

    $189.00

  • EasyFilter Plasmid Midiprep Kit (50 Preps)

    EasyFilter Plasmid Midiprep Kit (50 Preps)

    EasyPrep™ Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 100-250 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrepTM Plasmid Midiprep Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Midiprep Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD03-01PD03-11 PD03-02PD03-12 PD03-03PD03-13 Midiprep DNA Columns 10 25 50 EasyFilter 10(PD03-11) 25(PD03-12) 50(PD03-13) Buffer A1 30 ml 80 ml 160 ml Buffer A2 30 ml 80 ml 160 ml Buffer A3 45 ml 100 ml 250 ml Elution Buffer 10 ml 20 ml 40 ml Column Buffer 15 ml 30 ml 60 ml RNase A 110 µl 275 µl 540 µl 15ml conical tubes 10 25 25 Manual 1 1 1  

    $300.00

  • Sale -10% Endofree Plasmid Midiprep Kit (10 Preps) Endofree Plasmid Midiprep Kit (10 Preps)

    Endofree Plasmid Midiprep Kit (10 Preps)

    EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 Midiprep DNA Columns 10 25 EasyFilter 10(PD03-31) 25(PD03-32) Buffer A1 30 ml 75 ml Buffer B1 30 ml 75 ml Buffer N3 15 ml 30 ml Buffer RET 30 ml 75 ml DNA Wash Buffer (5x)* 25 ml 50 ml EndoFree Elution Buffer 10 ml 20 ml RNase A 110 µl 250 µl Manual 1 1 * Add ethanol to washing buffer before use

    $78.00 $70.00

  • Sale -15% Endofree Plasmid Midiprep Kit (25 Preps) Endofree Plasmid Midiprep Kit (25 Preps)

    Endofree Plasmid Midiprep Kit (25 Preps)

    EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 Midiprep DNA Columns 10 25 EasyFilter 10(PD03-31) 25(PD03-32) Buffer A1 30 ml 75 ml Buffer B1 30 ml 75 ml Buffer N3 15 ml 30 ml Buffer RET 30 ml 75 ml DNA Wash Buffer (5x)* 25 ml 50 ml EndoFree Elution Buffer 10 ml 20 ml RNase A 110 µl 250 µl Manual 1 1 * Add ethanol to washing buffer before use

    $189.00 $160.00

  • Endofree EasyFilter Plasmid Midiprep Kit (10 Preps) Endofree EasyFilter Plasmid Midiprep Kit (10 Preps)

    Endofree EasyFilter Plasmid Midiprep Kit (10 Preps)

    EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 Midiprep DNA Columns 10 25 EasyFilter 10(PD03-31) 25(PD03-32) Buffer A1 30 ml 75 ml Buffer B1 30 ml 75 ml Buffer N3 15 ml 30 ml Buffer RET 30 ml 75 ml DNA Wash Buffer (5x)* 25 ml 50 ml EndoFree Elution Buffer 10 ml 20 ml RNase A 110 µl 250 µl Manual 1 1 * Add ethanol to washing buffer before use

    $95.00

  • Endofree EasyFilter Plasmid Midiprep Kit (25 Preps) Endofree EasyFilter Plasmid Midiprep Kit (25 Preps)

    Endofree EasyFilter Plasmid Midiprep Kit (25 Preps)

    EasyPrep™ EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections. Kit's Contents SKU PD03-21PD03-31 PD03-22PD03-32 Midiprep DNA Columns 10 25 EasyFilter 10(PD03-31) 25(PD03-32) Buffer A1 30 ml 75 ml Buffer B1 30 ml 75 ml Buffer N3 15 ml 30 ml Buffer RET 30 ml 75 ml DNA Wash Buffer (5x)* 25 ml 50 ml EndoFree Elution Buffer 10 ml 20 ml RNase A 110 µl 250 µl Manual 1 1 * Add ethanol to washing buffer before use

    $225.00

  • Sale -6% Plasmid midiprep-II kit (10 Preps) Plasmid midiprep-II kit (10 Preps)

    Plasmid midiprep-II kit (10 Preps)

    EasyPrep™ Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 400-500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrepTM Plasmid Midiprep-II Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Midiprep-II Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD04-01PD04-11 PD04-02PD04-12 Midiprep DNA Columns 10 25 EasyFilter 10 (PD04-11) 25 (PD04-12) Buffer A1 60 ml 150 ml Buffer A2 60 ml 150 ml Buffer A3 75 ml 180 ml Elution Buffer 10 ml 20 ml Column Buffer 35 ml 80 ml RNase A 210 µl 520 µl 50ml conical tubes 10 25 Manual 1 1

    $69.00 $65.00

  • Sale -4% Plasmid midiprep-II kit (25 Preps)

    Plasmid midiprep-II kit (25 Preps)

    EasyPrep™ Plasmid Midiprep-II Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 400-500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrepTM Plasmid Midiprep-II Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Midiprep-II Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD04-01PD04-11 PD04-02PD04-12 Midiprep DNA Columns 10 25 EasyFilter 10 (PD04-11) 25 (PD04-12) Buffer A1 60 ml 150 ml Buffer A2 60 ml 150 ml Buffer A3 75 ml 180 ml Elution Buffer 10 ml 20 ml Column Buffer 35 ml 80 ml RNase A 210 µl 520 µl 50ml conical tubes 10 25 Manual 1 1  

    $156.00 $150.00

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