Purify DNA from enzymatic reactions (such as PCR) or chromatin immunoprecipitation (ChIP). Can not be used for gel extraction. Elution volume 35-50 ul. Kit's Contents SKU DC02-01 DC02-02 DNA mini Columns 50 250 Buffer PB* 25 ml 125 ml DNA Wash Buffer (Concentrate) 12 ml 54 ml Column Buffer 6 ml 30 ml Elution Buffer 10 ml 20 ml 2ml Collection Tubes 50 250 Manual 1 1 *Buffer PB contains chaotropic salts. Wear gloves and use protective eyewear when handling this solution. Buffer PB may form precipitates under cool ambient condition. Warm up the buffer at 37°C to dissolve before use.

Read more less

PowerPCR™ Pfu DNA polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. 2.5 U/µl  Amplify DNA target up to 2 kb The elongation velocity is 0.2~0.4kb/min (70~75°C). 6-10 fold higher fidelity than Taq DNA polymerase Highly thermostable – remains 95% active after 2 hours incubation at 95°C Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides) Possesses 3' to 5' exonuclease proofreading activity Generates in blunt-ended PCR products Applications High fidelity PCR Generation of PCR products for cloning and expression RT-PCR for cDNA cloning and expression Blunt-end PCR cloning

Read more less

Description Phi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29 and expressed by E. coli using gene recombination technology. This product has the ability of efficient continuous DNA synthesis and chain replacement, and has the function of 3 '→ 5' exonuclease reading, with high fidelity. This product can be applied to replication reactions requiring strong replacement and continuous synthesis and high fidelity replication under medium temperature conditions, such as plasmid replication, whole genome amplification, etc. Unit Definition At 30°C, within 10 minutes, the amount of enzyme required to add 0.5 pmol of deoxynucleotides to the acid insoluble precipitate is defined as 1 active unit (U). Thermal inactivation Incubation at 65°C for 10 minutes can inactivate. Quality Control After multiple column purification, the purity of SDS-PAGE was more than 95%; No endonuclease activity and no host residual DNA were detected. The enzyme buffer contains reductant DTT to ensure its maximum enzyme activity. If the buffer is not fresh or after repeated freezing and thawing, 4 mm DTT should be added before use. Storage Condition -20°C Components Phi29 DNA Polymerase, 10 U/μl 10x Phi29 Reaction Buffer BSA, 10 mg/ml

Read more less

The EasyPrep™ Plant genomic DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples.  Up to 100 mg of wet tissue (or 30 mg dry tissue) can be processed in less than 1 hour.  The system combines the reversible nucleic acid-binding properties of the matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates.  Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Kit's Contents SKU GD03-01 GD03-02 DNA Columns 50 250 Buffer PL1 40 ml 180 ml Buffer CPL1 30 ml 130 ml Buffer PL2 12 ml 50 ml Buffer PL3 15 ml 75 ml RNAse 100 µl 500 µl DNA Wash Buffer (5x) 20 ml 54 ml Elution Buffer 15 ml 60 ml Manual 1 1  

Read more less

The EasyPrep™ Plant genomic DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples.  Up to 100 mg of wet tissue (or 30 mg dry tissue) can be processed in less than 1 hour.  The system combines the reversible nucleic acid-binding properties of the matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates.  Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Kit's Contents SKU GD03-01 GD03-02 DNA Columns 50 250 Buffer PL1 40 ml 180 ml Buffer CPL1 30 ml 130 ml Buffer PL2 12 ml 50 ml Buffer PL3 15 ml 75 ml RNAse 100 µl 500 µl DNA Wash Buffer (5x) 20 ml 54 ml Elution Buffer 15 ml 60 ml Manual 1 1

Read more less

The EasyprepTM Plant total RNA kit provides an easy and fast method for isolating total RNA from plant tissues within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary. Kit's Contents SKU R03-01 R03-02 RNA Mini Columns 50 250 Buffer LY 28 mL 135 mL RB Buffer 25 mL 120 mL RNA Wash Buffer* 12 mL 50 mL DECP-Treated ddH2O 10 mL 30 mL Collection Tubes 100 500 DNase I (Optional)** 260 u 1300 u User Manual 1 1  

Read more less

The EasyprepTM Plant total RNA kit provides an easy and fast method for isolating total RNA from plant tissues within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary. Kit's Contents SKU R03-01 R03-02 RNA Mini Columns 50 250 Buffer LY 28 mL 135 mL RB Buffer 25 mL 120 mL RNA Wash Buffer* 12 mL 50 mL DECP-Treated ddH2O 10 mL 30 mL Collection Tubes 100 500 DNase I (Optional)** 260 u 1300 u User Manual 1 1

Read more less

EasyPrepTM Plasmid ezFilter Megaprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is ~3mg. Unlike all other rivals, our plasmid purification kit has no guanidine salt in the buffer, the purified DNA is guanidine/ion exchange resin residues free which enable the high performance of downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD06-01 PD06-02 Preps 2 10 Filter Units 2 10 Filter Unit Replacement Cup 2 10 DNA Binding Units 2 10 Buffer A1 80 ml 350 ml Buffer B1 80 ml 350 ml Buffer C1 100 ml 450 ml Elution Buffer 30 ml 150 ml RNase A 300 µl 1.1 ml Manual 1 1

Read more less

EasyPrepTM Plasmid ezFilter Megaprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is ~3mg. Unlike all other rivals, our plasmid purification kit has no guanidine salt in the buffer, the purified DNA is guanidine/ion exchange resin residues free which enable the high performance of downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD06-01 PD06-02 Preps 2 10 Filter Units 2 10 Filter Unit Replacement Cup 2 10 DNA Binding Units 2 10 Buffer A1 80 ml 350 ml Buffer B1 80 ml 350 ml Buffer C1 100 ml 450 ml Elution Buffer 30 ml 150 ml RNase A 300 µl 1.1 ml Manual 1 1

Read more less

EasyPrep™ Plasmid Maxiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is ≥500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrep™ Plasmid Maxiprep Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Maxiprep Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD05-01PD05-11 PD05-02PD05-12 Maxiprep DNA Columns 10 25 EasyFilter 10(PD05-11) 25(PD05-12) Buffer A1 120 ml 270 ml Buffer A2 120 ml 270 ml Buffer A3 130 ml 330 ml Elution Buffer 20 ml 30 ml Column Buffer 35 ml 85 ml RNase A 420 µl 920 µl 50ml conical tubes 10 25 Manual 1 1

Read more less

EasyPrep™ Plasmid Maxiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is ≥500 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming. EasyPrep™ Plasmid Maxiprep Kit applies DNA-binding-specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water. Plasmid DNAs purified from EasyPrep™ Plasmid Maxiprep Kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD05-01PD05-11 PD05-02PD05-12 Maxiprep DNA Columns 10 25 EasyFilter 10 (PD05-11) 25 (PD05-12) Buffer A1 120 ml 270 ml Buffer A2 120 ml 270 ml Buffer A3 130 ml 330 ml Elution Buffer 20 ml 30 ml Column Buffer 35 ml 85 ml RNase A 420 µl 920 µl 50ml conical tubes 10 25 Manual 1 1

Read more less