Description Phi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29 and expressed by E. coli using gene recombination technology. This product has the ability of efficient continuous DNA synthesis and chain replacement, and has the function of 3 '→ 5' exonuclease reading, with high fidelity. This product can be applied to replication reactions requiring strong replacement and continuous synthesis and high fidelity replication under medium temperature conditions, such as plasmid replication, whole genome amplification, etc. Unit Definition At 30°C, within 10 minutes, the amount of enzyme required to add 0.5 pmol of deoxynucleotides to the acid insoluble precipitate is defined as 1 active unit (U). Thermal inactivation Incubation at 65°C for 10 minutes can inactivate. Quality Control After multiple column purification, the purity of SDS-PAGE was more than 95%; No endonuclease activity and no host residual DNA were detected. The enzyme buffer contains reductant DTT to ensure its maximum enzyme activity. If the buffer is not fresh or after repeated freezing and thawing, 4 mm DTT should be added before use. Storage Condition -20°C Components Phi29 DNA Polymerase, 10 U/μl 10x Phi29 Reaction Buffer BSA, 10 mg/ml

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The EasyPrep™ Plant genomic DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples.  Up to 100 mg of wet tissue (or 30 mg dry tissue) can be processed in less than 1 hour.  The system combines the reversible nucleic acid-binding properties of the matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates.  Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Kit's Contents SKU GD03-01 GD03-02 DNA Columns 50 250 Buffer PL1 40 ml 180 ml Buffer CPL1 30 ml 130 ml Buffer PL2 12 ml 50 ml Buffer PL3 15 ml 75 ml RNAse 100 µl 500 µl DNA Wash Buffer (5x) 20 ml 54 ml Elution Buffer 15 ml 60 ml Manual 1 1  

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The EasyPrep™ Plant genomic DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples.  Up to 100 mg of wet tissue (or 30 mg dry tissue) can be processed in less than 1 hour.  The system combines the reversible nucleic acid-binding properties of the matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates.  Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. Kit's Contents SKU GD03-01 GD03-02 DNA Columns 50 250 Buffer PL1 40 ml 180 ml Buffer CPL1 30 ml 130 ml Buffer PL2 12 ml 50 ml Buffer PL3 15 ml 75 ml RNAse 100 µl 500 µl DNA Wash Buffer (5x) 20 ml 54 ml Elution Buffer 15 ml 60 ml Manual 1 1

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The EasyprepTM Plant total RNA kit provides an easy and fast method for isolating total RNA from plant tissues within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary. Kit's Contents SKU R03-01 R03-02 RNA Mini Columns 50 250 Buffer LY 28 mL 135 mL RB Buffer 25 mL 120 mL RNA Wash Buffer* 12 mL 50 mL DECP-Treated ddH2O 10 mL 30 mL Collection Tubes 100 500 DNase I (Optional)** 260 u 1300 u User Manual 1 1  

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The EasyprepTM Plant total RNA kit provides an easy and fast method for isolating total RNA from plant tissues within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary. Kit's Contents SKU R03-01 R03-02 RNA Mini Columns 50 250 Buffer LY 28 mL 135 mL RB Buffer 25 mL 120 mL RNA Wash Buffer* 12 mL 50 mL DECP-Treated ddH2O 10 mL 30 mL Collection Tubes 100 500 DNase I (Optional)** 260 u 1300 u User Manual 1 1

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EasyPrepTM Plasmid ezFilter Megaprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is ~10mg. Unlike all other rivals, our plasmid purification kit has no guanidine salt in the buffer, the purified DNA is guanidine/ion exchange resin residues free which enable the high performance of downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD06-05 PD06-06 Preps 2 10 Filter Units 2 10 Filter Unit Replacement Cup 2 10 DNA Binding Units 2 10 Buffer A1 210 ml 2x530 ml Buffer B1 210 ml 2x530 ml Buffer C1 250 ml 3x450 ml Elution Buffer 60 ml 270 ml RNase A 1.1ml 4x1.5 ml Manual 1 1

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EasyPrepTM Plasmid ezFilter Megaprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is ~10mg. Unlike all other rivals, our plasmid purification kit has no guanidine salt in the buffer, the purified DNA is guanidine/ion exchange resin residues free which enable the high performance of downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD06-05 PD06-06 Preps 2 10 Filter Units 2 10 Filter Unit Replacement Cup 2 10 DNA Binding Units 2 10 Buffer A1 210 ml 2x530 ml Buffer B1 210 ml 2x530 ml Buffer C1 250 ml 3x450 ml Elution Buffer 60 ml 270 ml RNase A 1.1ml 4x1.5 ml Manual 1 1

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EasyPrepTM Plasmid ezFilter Megaprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is ~3mg. Unlike all other rivals, our plasmid purification kit has no guanidine salt in the buffer, the purified DNA is guanidine/ion exchange resin residues free which enable the high performance of downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD06-01 PD06-02 Preps 2 10 Filter Units 2 10 Filter Unit Replacement Cup 2 10 DNA Binding Units 2 10 Buffer A1 80 ml 350 ml Buffer B1 80 ml 350 ml Buffer C1 100 ml 450 ml Elution Buffer 30 ml 150 ml RNase A 300 µl 1.1 ml Manual 1 1

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EasyPrepTM Plasmid ezFilter Megaprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is ~3mg. Unlike all other rivals, our plasmid purification kit has no guanidine salt in the buffer, the purified DNA is guanidine/ion exchange resin residues free which enable the high performance of downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD06-01 PD06-02 Preps 2 10 Filter Units 2 10 Filter Unit Replacement Cup 2 10 DNA Binding Units 2 10 Buffer A1 80 ml 350 ml Buffer B1 80 ml 350 ml Buffer C1 100 ml 450 ml Elution Buffer 30 ml 150 ml RNase A 300 µl 1.1 ml Manual 1 1

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EasyPrepTM Plasmid ezFilter Megaprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is ~5mg. Unlike all other rivals, our plasmid purification kit has no guanidine salt in the buffer, the purified DNA is guanidine/ion exchange resin residues free which enable the high performance of downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD06-05 PD06-06 Preps 2 10 Filter Units 2 10 Filter Unit Replacement Cup 2 10 DNA Binding Units 2 10 Buffer A1 130 ml 2x320 ml Buffer B1 130 ml 2x320 ml Buffer C1 160 ml 2x400 ml Elution Buffer 60 ml 300 ml RNase A 650 µl 2x1.6 ml Manual 1 1

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EasyPrepTM Plasmid ezFilter Megaprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is ~5mg. Unlike all other rivals, our plasmid purification kit has no guanidine salt in the buffer, the purified DNA is guanidine/ion exchange resin residues free which enable the high performance of downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations. Kit's Contents SKU PD06-05 PD06-06 Preps 2 10 Filter Units 2 10 Filter Unit Replacement Cup 2 10 DNA Binding Units 2 10 Buffer A1 130 ml 2x320 ml Buffer B1 130 ml 2x320 ml Buffer C1 160 ml 2x400 ml Elution Buffer 60 ml 300 ml RNase A 650 µl 2x1.6 ml Manual 1 1

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