Purify DNA from enzymatic reactions (such as PCR) or chromatin immunoprecipitation (ChIP). Can not be used for gel extraction. Elution volume 35-50 ul.
Kit's Contents
SKU
DC02-01
DC02-02
DNA mini Columns
50
250
Buffer PB*
25 ml
125 ml
DNA Wash Buffer (Concentrate)
12 ml
54 ml
Column Buffer
6 ml
30 ml
Elution Buffer
10 ml
20 ml
2ml Collection Tubes
50
250
Manual
1
1
*Buffer PB contains chaotropic salts. Wear gloves and use protective eyewear when handling this solution. Buffer PB may form precipitates under cool ambient condition. Warm up the buffer at 37°C to dissolve before use.
Purify DNA from enzymatic reactions (such as PCR) or chromatin immunoprecipitation (ChIP). Can not be used for gel extraction. Elution volume 35-50 ul.
Kit's Contents
SKU
DC02-01
DC02-02
DNA mini Columns
50
250
Buffer PB*
25 ml
125 ml
DNA Wash Buffer (Concentrate)
12 ml
54 ml
Column Buffer
6 ml
30 ml
Elution Buffer
10 ml
20 ml
2ml Collection Tubes
50
250
Manual
1
1
*Buffer PB contains chaotropic salts. Wear gloves and use protective eyewear when handling this solution. Buffer PB may form precipitates under cool ambient condition. Warm up the buffer at 37°C to dissolve before use.
PowerPCR™ Pfu DNA polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus.
2.5 U/µl
Amplify DNA target up to 2 kb
The elongation velocity is 0.2~0.4kb/min (70~75°C).
6-10 fold higher fidelity than Taq DNA polymerase
Highly thermostable – remains 95% active after 2 hours incubation at 95°C
Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Possesses 3' to 5' exonuclease proofreading activity
Generates in blunt-ended PCR products
Applications
High fidelity PCR
Generation of PCR products for cloning and expression
RT-PCR for cDNA cloning and expression
Blunt-end PCR cloning
Description
Phi29 DNA polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29 and expressed by E. coli using gene recombination technology. This product has the ability of efficient continuous DNA synthesis and chain replacement, and has the function of 3 '→ 5' exonuclease reading, with high fidelity. This product can be applied to replication reactions requiring strong replacement and continuous synthesis and high fidelity replication under medium temperature conditions, such as plasmid replication, whole genome amplification, etc.
Unit Definition
At 30°C, within 10 minutes, the amount of enzyme required to add 0.5 pmol of deoxynucleotides to the acid insoluble precipitate is defined as 1 active unit (U).
Thermal inactivation
Incubation at 65°C for 10 minutes can inactivate.
Quality Control
After multiple column purification, the purity of SDS-PAGE was more than 95%; No endonuclease activity and no host residual DNA were detected.
The enzyme buffer contains reductant DTT to ensure its maximum enzyme activity. If the buffer is not fresh or after repeated freezing and thawing, 4 mm DTT should be added before use.
Storage Condition
-20°C
Components
Phi29 DNA Polymerase, 10 U/μl
10x Phi29 Reaction Buffer
BSA, 10 mg/ml
The EasyPrep™ Plant genomic DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples. Up to 100 mg of wet tissue (or 30 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.
Kit's Contents
SKU
GD03-01
GD03-02
DNA Columns
50
250
Buffer PL1
40 ml
180 ml
Buffer CPL1
30 ml
130 ml
Buffer PL2
12 ml
50 ml
Buffer PL3
15 ml
75 ml
RNAse
100 µl
500 µl
DNA Wash Buffer (5x)
20 ml
54 ml
Elution Buffer
15 ml
60 ml
Manual
1
1
The EasyPrep™ Plant genomic DNA Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples. Up to 100 mg of wet tissue (or 30 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.
Kit's Contents
SKU
GD03-01
GD03-02
DNA Columns
50
250
Buffer PL1
40 ml
180 ml
Buffer CPL1
30 ml
130 ml
Buffer PL2
12 ml
50 ml
Buffer PL3
15 ml
75 ml
RNAse
100 µl
500 µl
DNA Wash Buffer (5x)
20 ml
54 ml
Elution Buffer
15 ml
60 ml
Manual
1
1
The EasyprepTM Plant total RNA kit provides an easy and fast method for isolating total RNA from plant tissues within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary.
Kit's Contents
SKU
R03-01
R03-02
RNA Mini Columns
50
250
Buffer LY
28 mL
135 mL
RB Buffer
25 mL
120 mL
RNA Wash Buffer*
12 mL
50 mL
DECP-Treated ddH2O
10 mL
30 mL
Collection Tubes
100
500
DNase I (Optional)**
260 u
1300 u
User Manual
1
1
The EasyprepTM Plant total RNA kit provides an easy and fast method for isolating total RNA from plant tissues within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary.
Kit's Contents
SKU
R03-01
R03-02
RNA Mini Columns
50
250
Buffer LY
28 mL
135 mL
RB Buffer
25 mL
120 mL
RNA Wash Buffer*
12 mL
50 mL
DECP-Treated ddH2O
10 mL
30 mL
Collection Tubes
100
500
DNase I (Optional)**
260 u
1300 u
User Manual
1
1
