The siRNA oligo can be labeled in the four different ends of double stranded by multiple markers. The labeled siRNA can be observed by flow cytometry, fluorescence microscopy, and laser scanning confocal microscope, which can determine whether the transfection is effective, and optimize transfection conditions. The labeled siRNA can also be used in siRNA intracellular localization and double labeling experiments (with labeled antibody) to track those siRNA transfected cells, then the reduction of target protein expression will be integrated with the transfection.
Labeling of the anti-sense 5’ end will influence the gene silencing activity, so labeling of this site is not recommended. Modification of any of other three ends has no influence on silencing activity. We recommend to modifying the 5’ end of sense strand, which is the best recognized chemical labeling locus.
Fluorescence-label includes 5'-FAM (green), 5'-Cy3 (red) and 3'-Cy3 (red).
The labeled siRNA oligos are in double stranded form (duplex), and purified by HPLC.
1 OD=2.5-3 nmol
Shipping cost for all siNRA orders is $30.
EasyPrep™ Fungal gDNA Miniprep Kits allow rapid and reliable isolation of high-quality total cellular DNA from a wide variety of Fungal species and tissues. Up to 100mg of wet tissue (or up to 50 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of Bioland’s matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.
Storage and Stability
All components of the EasyPrep™ Fungal DNA Miniprep Kit are stable for at least 12 months from date of purchase when stored at 22°C-25°C. During shipment or storage in cool ambient conditions, precipitates may form in Buffer FG 3. It is possible to dissolve such deposits by warming the solution at 37°C.
Kit's Contents
SKU
GD06-01
GD06-02
DNA Columns
50
250
Collection Tubes
100
500
Buffer FG1
40 mL
180 mL
Buffer FG2
12mL
50 mL
Buffer FG3
15mL
200 mL
Buffer CFG1
30 mL
130 mL
DNA Wash Buffer
12 mL
54 mL
Elution Buffer
15 mL
50 mL
RNase A
100 μL
500 μL
Manual
1
1
EasyPrep™ Fungal gDNA Miniprep Kits allow rapid and reliable isolation of high-quality total cellular DNA from a wide variety of Fungal species and tissues. Up to 100mg of wet tissue (or up to 50 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of Bioland’s matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.
Storage and Stability
All components of the EasyPrep™ Fungal DNA Miniprep Kit are stable for at least 12 months from date of purchase when stored at 22°C-25°C. During shipment or storage in cool ambient conditions, precipitates may form in Buffer FG 3. It is possible to dissolve such deposits by warming the solution at 37°C.
Kit's Contents
SKU
GD06-01
GD06-02
DNA Columns
50
250
Collection Tubes
100
500
Buffer FG1
40 mL
180 mL
Buffer FG2
12mL
50 mL
Buffer FG3
15mL
200 mL
Buffer CFG1
30 mL
130 mL
DNA Wash Buffer
12 mL
54 mL
Elution Buffer
15 mL
50 mL
RNase A
100 μL
500 μL
Manual
1
1
Extracting and purification of DNA fragment from agarose gels or enzymatic reactions (such as PCR).
Kit Contents:
SKU
DC01-01
DC01-02
DNA mini Columns
50
250
Buffer GB*
30 ml
130 ml
DNA Wash Buffer (Concentrate)
12 ml
54 ml
Column Buffer
6 ml
30 ml
Elution Buffer
10 ml
20 ml
2ml Collection Tubes
50
250
User Manual
1
1
*Buffer GB contains chaotropic salts. Wear gloves and use protective eyewear when handling this solution. Buffer GB may form precipitates under cool ambient condition. Warm up the buffer at 37°C to dissolve before use.
Extracting and purification of DNA fragment from agarose gels or enzymatic reactions (such as PCR).
Kit Contents:
SKU
DC01-01
DC01-02
DNA mini Columns
50
250
Buffer GB*
30 ml
130 ml
DNA Wash Buffer (Concentrate)
12 ml
54 ml
Column Buffer
6 ml
30 ml
Elution Buffer
10 ml
20 ml
2ml Collection Tubes
50
250
User Manual
1
1
*Buffer GB contains chaotropic salts. Wear gloves and use protective eyewear when handling this solution. Buffer GB may form precipitates under cool ambient condition. Warm up the buffer at 37°C to dissolve before use.
Dual-plate structure, compatible with BVE-4 electrophoresis system.
The electrophoresis tank is molded from high-transparency polycarbonate in a single injection process, featuring corrosion resistance, high transparency, and product stability.
The transfer clip have clearly marked positive and negative poles, equipped with handles for convenient operation.
High blotting efficiency, with a blotting time of approximately 20~40 minutes.Structurally stable materials allow for simultaneous ice bath cooling to prevent overheating interference and ensure reliable experimental results.
Capable of blotting four membranes simultaneously.
Blotting membrane size: 75x100mm
Gel Size
1-4
Gel Size
83 X 73mm
External Dimensions
140 x 180 x 185mm
Comb Specifications
1.0 mm : 11 teeth / 15 teeth
1.5mm: 11 teeth / 15 teeth
0.75 mm: 11 teeth / 15 teeth
Maximum Buffer Capacity
Genie AC Pack is designed as pre-purification pack for Genie water system to remove free chlorine from tap water. This protects the reverse osmosis (RO) membrane against chlorine oxidation.
Purification Technologies
Activated carbon for free chlorine reduction and bacteriostatic protection
Technical Specifications
Burst pressure
≥20bar
Flow rate
≥2L/min(Feed pressure ≥ 2 bar)
Height
393.8mm
Diameter
φ90mm
Suitable for Genie water systems
Genie G
Genie U
Genie E
Genie R
Genie Cleaning Pack used as cleaning pack for Genie water system to clean RO membrane.
Suitable for Genie water systems:
Genie G
Genie U
Genie A
Genie E
Genie R
Genie E Set
Feed: tap water
Product: Type II Water
System includes
Main system
Control Console
RO membrane(s)
Genie Cleaning Pack
30L tank and vent filter
UV preinstalled
Two cartridges
Leak protector
Dispensers are optional.
Feed Water Requirements
Tap Water
Municipal water, Conductivity < 2000 μS/cm (1000 ppm)
Operating temperature
5 – 35 ℃
Feed water pressure
1 – 6 bar psi (15 – 90)
Water Flow Rate
EDI water production rate (@ 25℃)
5 L/hr
Water dispensing rate
Up to 2.0 L/min
Product Water Quality
RO rejection
97 to 98% ionic rejection (new RO cartridge)> 99% organic rejection> 99% particulates & bacteria rejection
Water resistivity (@ 25℃)
> 5 MΩ·cm (typically 10 – 16 MΩ·cm)
TOC*
< 30 ppb
Product Water Quality Stored in Tank ( for tank recirculation models )
Resistivity (@25℃)
> 1 MΩ·cm (default), adjustable
Dimensions
Main system dimensionsWidth × depth × height
32 × 44 × 54 cm
(12.6 × 17.3 × 21.3 in)
Dispenser dimensions
Width × depth × height
21 × 29 × 61 cm
(8.3 × 11.4 × 24.0 in)
Weight
20 kg
Input Voltage
110 – 240 VAC
Operating Voltage
24 VDC
Main system power
< 200 W
*Product water quality may vary due to local feed water conditions.
Genie G Set
Feed: tap water
Product: Type II water and Type I
System includes
Main system with TOC
Control Console
One Dispenser
RO membrane(s)
UV preinstalled
Three cartridges and a 0.2 um final filter
30L tank
Tank vent filter
Leak protector
More dispensers are optional
Feed Water Requirements
Tap Water
Municipal water, Conductivity < 2000 μS/cm (1000 ppm)
Operating temperature
5 – 35 ℃
Feed water pressure
1 – 6 bar (15 – 90 psi)
Water Flow Rate
EDI water production rate (@ 25℃)
5, 10, 15 L/hr
EDI water dispensing rate
Up to 2.0 L/min
Ultrapure water dispensing rate
Up to 2.0 L/min
Product Water Quality
RO rejection
97 to 98% ionic rejection (new RO cartridge)> 99% organic rejection> 99% particulates & bacteria rejection
EDI water resistivity (@ 25℃)
> 5 MΩ·cm (typically 10 – 16 MΩ·cm)
EDI water TOC*
< 30 ppb
Ultrapure water resistivity (@ 25℃)
18.2 MΩ·cm
Ultrapure water TOC*
< 2 ppb
Particles in ultrapure water
( > 0.2 μm)
No particles (with a 0.2 μm final filter or RephiBio filter)
Microorganisms in ultrapure water
< 0.001 cfu/ml (with a 0.2 μm final filter or RephiBio filter)
Pyrogens in ultrapure water
< 0.001 Eu/ml (with a RephiBio filter)
RNase in ultrapure water
< 0.5 pg/ml (with a RephiBio filter)
DNase in ultrapure water
<10 pg/ml (with a RephiBio filter)
Product Water Quality Stored in Tank ( for tank recirculation models )
Resistivity (@25℃)
> 1 MΩ·cm (default), adjustable
Dimensions
Main system dimensionsWidth × depth × height
32 × 44 × 54 cm (12.6 × 17.3 × 21.3 in)
Dispenser dimensions
Width × depth × height
21 × 29 × 61 cm (8.3 × 11.4 × 24.0 in)
Weight
20 kg
Input Voltage
110 – 240 VAC
Operating Voltage
24 VDC
*In the appropriate operating conditions, otherwise typically ≤5 ppb.
Genie H Pack is high-purification pack for Genie water system to remove ionic impurities from water.
Purification Technologies:
Ion-exchange resins for removing ionic contaminants
Suitable for Genie water systems:
Genie U
Genie PURIST