The EasyPrepTM total RNA kit provides an easy and fast method for isolating total RNA from Gram-positive (B. subtilis) Or Gram-negative (E. coli) Bacteria within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary. Storage and Stability DNase I (optional) and lysozyme should be stored at -20°C. All other components can be stored at room temperature. All kit components are guaranteed for 12 monthsr from the date of purchasing. Kit's Contents SKU R04-01 R04-02 Preps 50 250 Buffer LY 28 mL 135 mL Buffer RB 30 mL 135 mL RNA Wash Buffer Concentrate* 20 mL 3 x 24 mL Elution Buffer (DECP-Treated ddH2O) 10 mL 30 mL DNase Stop Buffer 2.4 mL 12 mL Collection Tubes 100 500 Lysozyme 15 mg 75 mg User Manual 1 1  Note: DNase I are not supplied. They could be purchased from us separately. Before Starting Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps. Important  Add 1 % volume of β-mercaptoethanol to Buffer LY before use and store at 4°C.  Add 80 mL (R04-01) or 96 mL (R04-02) 100% ethanol to RNA Wash Buffer before use.  Add 9.6 mL (R04-01) or 48 mL (R04-02) 100% ethanol to DNase Stop Buffer before use.  Prepare a lysozyme stock solution at 3 mg/mL or 0.4 mg/mL with Elution Buffer or TE Buffer and aliquot into adequate portions. Store each aliquot at -20°C and thaw before use.

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The EasyPrepTM total RNA kit provides an easy and fast method for isolating total RNA from Gram-positive (B. subtilis) Or Gram-negative (E. coli) Bacteria within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary. Storage and Stability DNase I (optional) and lysozyme should be stored at -20°C. All other components can be stored at room temperature. All kit components are guaranteed for 12 monthsr from the date of purchasing. Kit's Contents SKU R04-01 R04-02 Preps 50 250 Buffer LY 28 mL 135 mL Buffer RB 30 mL 135 mL RNA Wash Buffer Concentrate* 20 mL 3 x 24 mL Elution Buffer (DECP-Treated ddH2O) 10 mL 30 mL DNase Stop Buffer 2.4 mL 12 mL Collection Tubes 100 500 Lysozyme 15 mg 75 mg User Manual 1 1  Note: DNase I are not supplied. They could be purchased from us separately. Before Starting Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps. Important  Add 1 % volume of β-mercaptoethanol to Buffer LY before use and store at 4°C.  Add 80 mL (R04-01) or 96 mL (R04-02) 100% ethanol to RNA Wash Buffer before use.  Add 9.6 mL (R04-01) or 48 mL (R04-02) 100% ethanol to DNase Stop Buffer before use.  Prepare a lysozyme stock solution at 3 mg/mL or 0.4 mg/mL with Elution Buffer or TE Buffer and aliquot into adequate portions. Store each aliquot at -20°C and thaw before use.

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If you have all kinds of buffers and solutions in 15 ml and 50 ml tubes on your bench, this is for you! 30 positions for 5ml tubes 25 positions for 50ml tubes Pleasant color design

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If you have all kinds of buffers and solutions in 15 ml and 50 ml tubes on your bench, this is for you! Holding 30x 15ml tubes and 25x 50ml tubes. Pleasant color design.

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Grade: AR; For Molecular Biology CAS No.: 150-25-4 Synonym: N,N-Bis(2-Hydroxyethyl)glycine Molecular Fomula: C6H13NO4 Molecular Weight: 163.17 g/mol Storage condition: Room temperature Analytical Specifications Assay 100.2% Loss of Drying 0.12% pH (1.0% Aqueous) 4.3-5.3 Solubility (0.1M Aqueous) Clear, colorless Ultraviolet Absorbance (0.1M Aqueous) A280≤0.04A280≤0.03 Safety Data Sheet MSDS AR Grade- The standard Mallinckrodt grade of analytical reagents; suitable for laboratory and general use. Analytical Research Grade chemicals are a very high grade of chemical suitable for the specialist analytical testing carried out in laboratories where a high degree of accuracy is required.

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Grade: AR; For Molecular Biology CAS No.: 150-25-4 Synonym: N,N-Bis(2-Hydroxyethyl)glycine Molecular Fomula: C6H13NO4 Molecular Weight: 163.17 g/mol Storage condition: Room temperature Analytical Specifications Assay 100.2% Loss of Drying 0.12% pH (1.0% Aqueous) 4.3-5.3 Solubility (0.1M Aqueous) Clear, colorless Ultraviolet Absorbance (0.1M Aqueous) A280≤0.04A280≤0.03 Safety Data Sheet MSDS AR Grade- The standard Mallinckrodt grade of analytical reagents; suitable for laboratory and general use. Analytical Research Grade chemicals are a very high grade of chemical suitable for the specialist analytical testing carried out in laboratories where a high degree of accuracy is required.

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Grade: AR; For Molecular Biology CAS No.: 150-25-4 Synonym: N,N-Bis(2-Hydroxyethyl)glycine Molecular Fomula: C6H13NO4 Molecular Weight: 163.17 g/mol Storage condition: Room temperature Analytical Specifications Assay 100.2% Loss of Drying 0.12% pH (1.0% Aqueous) 4.3-5.3 Solubility (0.1M Aqueous) Clear, colorless Ultraviolet Absorbance (0.1M Aqueous) A280≤0.04A280≤0.03 Safety Data Sheet MSDS AR Grade- The standard Mallinckrodt grade of analytical reagents; suitable for laboratory and general use. Analytical Research Grade chemicals are a very high grade of chemical suitable for the specialist analytical testing carried out in laboratories where a high degree of accuracy is required.

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Grade: AR; For Molecular Biology CAS No.: 150-25-4 Synonym: N,N-Bis(2-Hydroxyethyl)glycine Molecular Fomula: C6H13NO4 Molecular Weight: 163.17 g/mol Storage condition: Room temperature Analytical Specifications Assay 100.2% Loss of Drying 0.12% pH (1.0% Aqueous) 4.3-5.3 Solubility (0.1M Aqueous) Clear, colorless Ultraviolet Absorbance (0.1M Aqueous) A280≤0.04A280≤0.03 Safety Data Sheet MSDS AR Grade- The standard Mallinckrodt grade of analytical reagents; suitable for laboratory and general use. Analytical Research Grade chemicals are a very high grade of chemical suitable for the specialist analytical testing carried out in laboratories where a high degree of accuracy is required.

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The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use the included CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch System is designed to advance your qPCR. Key Features and Benefits With the CFX96 Touch Real-Time PCR Detection System you can: Set up your system quickly — easy installation and factory-calibrated optics Minimize sample and reagent use — up to 5-target multiplexing with sample volumes as low as 10 µl Optimize reactions in a single run — thermal gradient feature Analyze data faster — visualize all run data at once and export only the data you need in the format you want Use advanced data analysis tools — normalized gene expression using CFX Maestro Software Configure the system to fit your needs — run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX Automation System II for higher throughput Combine the CFX96 Touch System with good laboratory practice standards — use CFX Maestro Software, Security Edition for data collection and analysis to simplify compliance with U.S. FDA 21 CFR Part 11 regulations Temperature 15-31°C Humidity <80% RH Altitude <2000 meters above sea level  

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Bio-Speedy® Respiratory Tract 4T RT-qPCR Kit is a one-step reverse transcription and real-time PCR (RT-qPCR) test intended for the qualitative detection of the RNA from SARS-CoV-2, Influenza A, Influenza B and Respiratory Syncytial Virus A/B. The Bio-Speedy® Respiratory Tract 4T RT-qPCR Kit is applied to nucleic acids obtained from nasopharyngeal swab, oropharyngeal swab, oral/saliva swab, bronchoalveolar lavage, nasopharyngeal aspirate, saliva, gargle, and sputum samples.Detection with the kit is achieved via rapid nucleic acid extraction from nasopharyngeal swab, oropharyngeal swab, oral/saliva swab, bronchoalveolar lavage, nasopharyngeal aspirate, saliva, gargle, and sputum samples followed by multiplex RT-q PCR targeting the SARS-CoV-2-specific Open Reading Frame 1ab (ORF1ab), Nucleocapsid (N), Influenza A-specific Membrane Protein (M), Influenza B-specific Nuclear Export Protein (NEP), Respiratory Syncytial Virus A/B-specific Nucleocapsid (N) genes in real-time PCR instruments that are equipped with FAM, HEX, ROX, CY5 and CY5.5 detection channels. The kit allows to achieve RT-qPCR results in less than 30 minutes (Run time may vary depending on the instrument and the thermal cycling protocol).The oligonucleotide set targeting the human RNase P mRNA functions as a control of the sampling, nucleic acid extraction, reverse transcription, and qPCR since the oligonucleotide set targets the exon-exon junction. The kit also contains negative and positive control templates for testing the contamination and the RT-qPCR reagent stability, respectively.Bio-Speedy® Respiratory Tract 4T RT-qPCR Kit is intended for use by laboratory personnel trained in the techniques of RT-qPCR. RUO

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Bio-Speedy® Respiratory Tract 4T RT-qPCR Kit is a one-step reverse transcription and real-time PCR (RT-qPCR) test intended for the qualitative detection of the RNA from SARS-CoV-2, Influenza A, Influenza B and Respiratory Syncytial Virus A/B. The Bio-Speedy® Respiratory Tract 4T RT-qPCR Kit is applied to nucleic acids obtained from nasopharyngeal swab, oropharyngeal swab, oral/saliva swab, bronchoalveolar lavage, nasopharyngeal aspirate, saliva, gargle, and sputum samples. Detection with the kit is achieved via rapid nucleic acid extraction from nasopharyngeal swab, oropharyngeal swab, oral/saliva swab, bronchoalveolar lavage, nasopharyngeal aspirate, saliva, gargle, and sputum samples followed by multiplex RT-q PCR targeting the SARS-CoV-2-specific Open Reading Frame 1ab (ORF1ab), Nucleocapsid (N), Influenza A-specific Membrane Protein (M), Influenza B-specific Nuclear Export Protein (NEP), Respiratory Syncytial Virus A/B-specific Nucleocapsid (N) genes in real-time PCR instruments that are equipped with FAM, HEX, ROX, CY5 and CY5.5 detection channels. The kit allows to achieve RT-qPCR results in less than 30 minutes (Run time may vary depending on the instrument and the thermal cycling protocol). The oligonucleotide set targeting the human RNase P mRNA functions as a control of the sampling, nucleic acid extraction, reverse transcription, and qPCR since the oligonucleotide set targets the exon-exon junction. The kit also contains negative and positive control templates for testing the contamination and the RT-qPCR reagent stability, respectively. Bio-Speedy® Respiratory Tract 4T RT-qPCR Kit is intended for use by laboratory personnel trained in the techniques of RT-qPCR. RUO  

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Direct RT-qPCR SARS-CoV-2 Intended use: Bio-Speedy® Direct RT-qPCR SARS-CoV-2 kit is a real-time and one-step reverse transcription PCR test used to detect the SARS-CoV-2-specific ORF1ab gene qualitatively. The vNAT® Buffer included in the kit obtains and preserves nucleic acid extract from the samples in VTM tubes. Targets: The Bio-Speedy® Direct RT-qPCR SARS-CoV-2 kit targets the ORF1ab gene in the SARS-CoV-2 genome and is read in the FAM channel of the Real-Time PCR instrument. The RNase P gene targeted as an internal control is also read in the HEX channel. Operation and Duration: Bio-Speedy® Direct RT-qPCR SARS-CoV-2 kit makes samples taken in VTM ready for Real-Time PCR within 5 minutes after combining them with vNAT® Buffer and provides Real-Time PCR results in 55 minutes.  Validated and Compatible Devices: The kit is validated for 10 and 20 µL qPCR volumes using Roche LightCycler® 96, Bio-Rad CFX96 Touch™, and Qiagen Rotor-Gene® 5 Plex Real-Time PCR Systems. Sensitivity: 97.8%Specificity: 100% The Bio-Speedy® Direct RT-qPCR SARS-CoV-2 kit is an Emergency Use Authorization (EUA) kit authorized by the U.S. FDA for use by licensed laboratories. IFU  

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