Molecular cloning assisted by vectors is the most popular and common method to obtain genes of interest. Yeastern Biotech’s T&A™ Cloning Kit offers a quick, reliable and efficient method for cloning a variety of DNA sequences.
The T&A™ Cloning Kit contains the T&A™ Cloning Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3′-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™) or be purified first to achieve higher transformation efficiency.
Features
- Fast ligation, completed in only 5 minutes
- More accurate results
- Accept a wide range of inserts with different sizes
- Two types of ligation buffers provided for your convenience
- Allow blue/white screening
- Contain ampicillin marker for antibiotic selection
- Include M13 primer sites for convenient sequencing
Applications
- Cloning of terminal 3’-dA nucleotides overhang PCR products up to 5 kb
- Quality Control
- DNA concentration of T&A™ Cloning Vector is 25 ng/μl
- The absorbance ratio (A260/A280) is between 1.6~2.0
- The size of T&A™ Cloning Vector is about 2.7 kb
- The colony number of background control is less than 50 when the transformation efficiency of competent cells is 1 × 108cfu/μg DNA
- The colony number ratio of self-ligation control to positive control is less than 15%
- The colony number of the positive control is more than 500 when the transformation efficiency of competent cells is 5 × 108cfu/μg DNA
- The ligation correctness with the control insert into T&A™ Cloning Vector is more than 87.5%
Store At
| T&A™ Cloning Vector |
40 μl (25 ng/ μl) |
| Control Insert DNA |
10 μl (10 ng/ μl) |
| yT4 DNA Ligase |
20 μl (2U/ μl) |
| 10X Ligation Buffer A |
50 μl |
| 10X Ligation Buffer B |
50 μl |
| Forward Primer (M13-F) |
50 μl (10 μM) |
| Reverse Primer (M13-R) |
50 μl (10 μM) |
***** Warning ! This is cold item, product requires to select Overnight shipping! *****
