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Klenow Fragment (3'→5' exo-), (5U/ul, 500U)

    Description

    DNA Pol I Large fragment (Klenow) was originally derived as a proteolytic product of E. coli DNA polymerase. It retains polymerase activity, but lacks both 5’ —> 3’ and 3' —> 5’ exonuclease activity.

    • Generates probes using random primers
    • Dideoxy sequencing
    • Random priming labeling
    • Moderate strand displacement activity
    • Not suitable for generating blunt ends

    Product Notes

    1. Klenow Fragment (3´→ 5´ exo-) is not suitable for generating blunt ends because it lacks the 3´→ 5´ exonuclease necessary to remove non-templated 3´ additions.
    2. When Klenow Fragment (3´→ 5´ exo-) is used to sequence DNA using the dideoxy method of Sanger et al., 1 unit/5 µl reaction volume is recommended.

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    Product form

    SKU: KL02-01

    Klenow Fragment (3'→5' exo-), (5U/ul, 500U)

      DNA Pol I Large fragment (Klenow) was originally derived as a proteolytic product of E. coli DNA polymerase. It retains... Read more

      $105.00

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        Description

        DNA Pol I Large fragment (Klenow) was originally derived as a proteolytic product of E. coli DNA polymerase. It retains polymerase activity, but lacks both 5’ —> 3’ and 3' —> 5’ exonuclease activity.

        • Generates probes using random primers
        • Dideoxy sequencing
        • Random priming labeling
        • Moderate strand displacement activity
        • Not suitable for generating blunt ends

        Product Notes

        1. Klenow Fragment (3´→ 5´ exo-) is not suitable for generating blunt ends because it lacks the 3´→ 5´ exonuclease necessary to remove non-templated 3´ additions.
        2. When Klenow Fragment (3´→ 5´ exo-) is used to sequence DNA using the dideoxy method of Sanger et al., 1 unit/5 µl reaction volume is recommended.

        Read more

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