Free Shipping for orders over $500!

Klenow Fragment (3'→5' exo-), (5U/ul, 500U)

    Description

    DNA Pol I Large fragment (Klenow) was originally derived as a proteolytic product of E. coli DNA polymerase. It retains polymerase activity, but lacks both 5’ —> 3’ and 3' —> 5’ exonuclease activity.

    • Generates probes using random primers
    • Dideoxy sequencing
    • Random priming labeling
    • Moderate strand displacement activity
    • Not suitable for generating blunt ends

    Product Notes

    1. Klenow Fragment (3´→ 5´ exo-) is not suitable for generating blunt ends because it lacks the 3´→ 5´ exonuclease necessary to remove non-templated 3´ additions.
    2. When Klenow Fragment (3´→ 5´ exo-) is used to sequence DNA using the dideoxy method of Sanger et al., 1 unit/5 µl reaction volume is recommended.

    Read more

    Product form

    SKU: KL02-01

    Klenow Fragment (3'→5' exo-), (5U/ul, 500U)

      DNA Pol I Large fragment (Klenow) was originally derived as a proteolytic product of E. coli DNA polymerase. It retains... Read more

      $105.00

      Free pickup in our shop(s)
            Want a Bulk Order? Contact us!

          Our team is available Monday through Friday from 9:00am-5:30pm PST.

          Send us a message!

          Description

          DNA Pol I Large fragment (Klenow) was originally derived as a proteolytic product of E. coli DNA polymerase. It retains polymerase activity, but lacks both 5’ —> 3’ and 3' —> 5’ exonuclease activity.

          • Generates probes using random primers
          • Dideoxy sequencing
          • Random priming labeling
          • Moderate strand displacement activity
          • Not suitable for generating blunt ends

          Product Notes

          1. Klenow Fragment (3´→ 5´ exo-) is not suitable for generating blunt ends because it lacks the 3´→ 5´ exonuclease necessary to remove non-templated 3´ additions.
          2. When Klenow Fragment (3´→ 5´ exo-) is used to sequence DNA using the dideoxy method of Sanger et al., 1 unit/5 µl reaction volume is recommended.

          Read more

          Recently viewed products

            Login

            Forgot your password?

            Don't have an account yet?
            Create account