DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).
Features
Suitable for cloning with large plasmid and cDNA library construction, and also
allows for blue-white colony screening.
Applications
- Cloning and subcloning
- Scale-up application
- Blue/white screening
Store at
Genotype
endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk–, mk+) ;supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ– F–
| Efficiency >1x109cfu/µg |
Item |
| 100 µl/vial |
80 vials |
| Control Plasmid (pUC19) |
|
***** Warning ! This is cold item, product requires to select Overnight shipping! *****
![ECOS X 1-Step Competent Cells [DH5a, 80x100ul]](http://bioland-sci.com/cdn/shop/files/ECOS_FyE607_101-Competent-Cells_38b35100-a3b4-4069-9cdd-da6089e36cd5.jpg?v=1757100089)