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ECOS 2163 1-Step Competent Cells [GM2163, 10x100ul]

    Description

    GM2163 is an E. coli K12 strain, which is deficient in both dam and dcm genes, is suitable for the propagation of plasmid or cloned DNA to be cut with Dam or Dcm-sensitive restriction enzymes.

    This strain is not recommended as a host for primary cloning/ligation since the dam mutation can result in higher mutation rates and a reduction in the transformation efficiency.

    Features

    • Chloramphenicol resistant
    • Not suitable for blue/white screening
    • Not recommended as a host for primary cloning/ligation

    Applications

    • Propagation of plasmid-free of Dam and Dcm methylations

    Store At

    Genotype

    F–ara–14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm–6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl–1 thi-1 mcrB1 hsdR2

    ***** Warning ! This is cold item, product requires to select Overnight shipping! *****

    Read more

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    SKU: FYE807-10VL

    ECOS 2163 1-Step Competent Cells [GM2163, 10x100ul]

      GM2163 is an E. coli K12 strain, which is deficient in both dam and dcm genes, is suitable for the... Read more

      $335.00

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          Description

          GM2163 is an E. coli K12 strain, which is deficient in both dam and dcm genes, is suitable for the propagation of plasmid or cloned DNA to be cut with Dam or Dcm-sensitive restriction enzymes.

          This strain is not recommended as a host for primary cloning/ligation since the dam mutation can result in higher mutation rates and a reduction in the transformation efficiency.

          Features

          • Chloramphenicol resistant
          • Not suitable for blue/white screening
          • Not recommended as a host for primary cloning/ligation

          Applications

          • Propagation of plasmid-free of Dam and Dcm methylations

          Store At

          Genotype

          F–ara–14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm–6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl–1 thi-1 mcrB1 hsdR2

          ***** Warning ! This is cold item, product requires to select Overnight shipping! *****

          Read more

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