This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
Genotype
F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Features
- Lack of Lon and Omp proteases allows high-level protein expression and accumulation
- Easy gene induction
Applications
- IPTG induced gene expression in E. coli
- Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
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Subcloning
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![ECOS 21 1-Step Competent Cells [BL21, 80x100ul]](http://bioland-sci.com/cdn/shop/files/ECOS_FyE607_101-Competent-Cells_38b35100-a3b4-4069-9cdd-da6089e36cd5.jpg?v=1757100089)