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ECOS 21 1-Step Competent Cells [BL21, 10x100ul]

    Description

    This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.

    Genotype

    F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

    Features

    • Lack of Lon and Omp proteases allows high-level protein expression and accumulation
    • Easy gene induction

    Applications

    • IPTG induced gene expression in E. coli
    • Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
    • Subcloning

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    ***** Warning ! This product requires to select Overnight shipping. *****

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    SKU: FYE207-10VL

    ECOS 21 1-Step Competent Cells [BL21, 10x100ul]

      This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase... Read more

      $150.00

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        Description

        This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.

        Genotype

        F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

        Features

        • Lack of Lon and Omp proteases allows high-level protein expression and accumulation
        • Easy gene induction

        Applications

        • IPTG induced gene expression in E. coli
        • Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
        • Subcloning

        Store At:

        ***** Warning ! This product requires to select Overnight shipping. *****

        Read more

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