DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).
Features
Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.
Applications
- Cloning and subcloning
- Scale-up application
- Blue/white screening
Store at
Genotype
endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk–, mk+) supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ– F–
| Efficiency >1x108cfu/µg |
Item |
| 100 µl/vial |
10 vials |
| Control Plasmid (pUC19) |
5 μl (10-4μg/μl) |
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