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ECOS 101 1-Step Competent Cells [DH5a, 10x100ul]

    Description

    DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).

    Features

    Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.

    Applications

    • Cloning and subcloning
    • Scale-up application
    • Blue/white screening

    Store at

    Genotype

    endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk–, mk+) supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ– F–

    Efficiency >1x108cfu/µg Item
    100 µl/vial 10 vials
    Control Plasmid (pUC19) 5 μl (10-4μg/μl)

     

    ***** This is a cold item. You are requires to select Overnight shipping for purchase this item! *****

    Read more

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    SKU: FYE607-10VL

    ECOS 101 1-Step Competent Cells [DH5a, 10x100ul]

      DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of... Read more

      $150.00

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          Description

          DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).

          Features

          Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.

          Applications

          • Cloning and subcloning
          • Scale-up application
          • Blue/white screening

          Store at

          Genotype

          endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk–, mk+) supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ– F–

          Efficiency >1x108cfu/µg Item
          100 µl/vial 10 vials
          Control Plasmid (pUC19) 5 μl (10-4μg/μl)

           

          ***** This is a cold item. You are requires to select Overnight shipping for purchase this item! *****

          Read more

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