Bio-Speedy® Respiratory Tract 4T RT-qPCR Kit is a one-step reverse transcription and real-time PCR (RT-qPCR) test intended for the qualitative detection of the RNA from SARS-CoV-2, Influenza A, Influenza B and Respiratory Syncytial Virus A/B. The Bio-Speedy® Respiratory Tract 4T RT-qPCR Kit is applied to nucleic acids obtained from nasopharyngeal swab, oropharyngeal swab, oral/saliva swab, bronchoalveolar lavage, nasopharyngeal aspirate, saliva, gargle, and sputum samples.
Detection with the kit is achieved via rapid nucleic acid extraction from nasopharyngeal swab, oropharyngeal swab, oral/saliva swab, bronchoalveolar lavage, nasopharyngeal aspirate, saliva, gargle, and sputum samples followed by multiplex RT-q PCR targeting the SARS-CoV-2-specific Open Reading Frame 1ab (ORF1ab) , Nucleocapsid (N) , Influenza A-specific Membrane Protein (M) , Influenza B-specific Nuclear Export Protein (NEP) , Respiratory Syncytial Virus A/B-specific Nucleocapsid (N) genes in real-time PCR instruments that are equipped with FAM , HEX , ROX , CY5 and CY5.5 The kit allows to achieve RT-qPCR results in less than 30 minutes (Run time may vary depending on the instrument and the thermal cycling protocol).
The oligonucleotide set targeting the human RNase P mRNA functions as a control of the sampling, nucleic acid extraction, reverse transcription, and qPCR since the oligonucleotide set targets the exon-exon junction. The kit also contains negative and positive control templates for testing the contamination and the RT-qPCR reagent stability, respectively.
Bio-Speedy® Respiratory Tract 4T RT-qPCR Kit
