- 3 μl or 5 μl per loading for clear visualization during electrophoresis
on 15-well or 10-well mini-gel, respectively.
- 1.5~2.5 μl per well for general Western transferring.
- Apply more for thicker (> 1.5 mm) or larger gel.
- The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, or add reducing agent before loading.
Description: The All Blue Prestained Protein Ladder I is a protein standard with 10 pre-stained proteins covering a wide range molecular weightsfor 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore and two reference bands ( 25 kDa and 72 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The All Blue Prestained Protein Ladder I is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.
Contents: Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM 2-mercaptoethanol, 3.6M Urea, and 15 % (v/v) Glycerol).
Quality Control: Under suggested conditions, All Blue Prestained Protein Ladder I resolves10 major bands in polyacrylamide gel with appropriate buffers andafterWestern blotting to nitrocellulose membrane.
Storage:
- Stable at 25°C for 2 weeks
- Stable at 4°C for 3 months
- Stable at -20°C for 24 months
