5X All-In-One 1st Strand cDNA Synthesis Mix is an optimized ready-to-use mastermix, which eliminates multiple component additions. The mastermix provides sensitive and reliable cDNA synthesis over a range of input RNAs. The size of target cDNA is up to 9kb.
Composition: PowerScript™ Reverse transcriptase, Ribonuclease inhibitor, dNTPs, Oligo(dT)s, Random primers in optimized reaction buffer.
PowerScript™ Reverse Transcriptase: a specially engineered RTase based on native RTase from MMLV, (1) No RNase H activity to prevent RNA degradation; (2) Contains a fidelity-enhancing subunit to ensure accuracy in reverse transcription.
Size: 200 µl; 3x1ml Nuclease-free H2O
Storage: -20ºC
Applications
- Generation of templates for RT-PCR and qPCR
- cDNA synthesis from ssRNA
- cDNA library construction
- Generation of probes for hybridization
- DNA primer extension
