For 250 preps: 125 ml Buffer PB, 50 ml DNA Wash Buffer concentrate (for 1x250 ml) and 20 ml Elution Buffer. 

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With 60 ml capacity, good for plasmid purification maxiprep kits. 10 pieces

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Good for plasmid miniprep, midiprep and maxiprep kits.

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Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide. Specific Activity: 40 U/mg protein Molecular Weight: 28.9 kDa monomer Source: Pichia pastoris cells with a cloned gene encoding Tritirachium album endolytic protease (Proteinase K). Applications Isolation of genomic DNA from cultured cells and tissues Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines  Determination of enzyme localization Improving cloning efficiency of PCR products  Quality Control DNase Activity: None detectable enzyme activity with λ DNA after 6 hrs incubation at 37ºC. RNase Activity: None detectable ribonuclease activity after 16 hrs incubation at 25ºC.  Dilution Buffer 50 mM Tris-HCl (pH 7.5), containing 5 mM calcium chloride and 50% (v/v) glycerol.  Definition of Activity Unit One unit of the enzyme liberates Folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 min at 37°C using denatured hemoglobin as substrate.Enzyme activity is assayed in the following mixture: 0.08 M potassium phosphate (pH 7.5), 5 M urea, 4 mM NaCl, 3 mM CaCl2 and 16.7 mg/ml hemoglobin. Storage For long time storage, store the Proteinase K powder at 4ºC and solution at -20°C.  Inhibition and Inactivation Inhibitors: Proteinase K is not inactivated by metal chelators, by thiol-reactive reagents or by specific trypsin and chymotrypsin inhibitors. Phenylmethylsulfonyl fluoride and diisopropyl phosphorofluoridate completely inhibit the enzyme. Inactivated by heating at 95°C for 10 minutes.  Other Note: Optimum activity at 50-55°C.  Rapid denaturation of enzyme occurs at temperatures above 65°C.  The recommended working concentration for Proteinase K is 0.05-1 mg/ml. The activity of the enzyme is stimulated by 0.2-1% SDS or by 1-4 M urea. Ca2+ protects Proteinase K against autolysis, increases the thermal stability and has a regulatory function for the substrate binding site of Proteinase K. Stable over a wide pH range: 4.0-12.5, optimum pH 7.5-8.0.

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Good for 250 RNA extraction minipreps

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 For 250 preps: 80 ml A1, 80 ml A2, 110 ml N1, 50 ml washing buffer concentrate (for 250 ml washing buffer), 20 ml Elution buffer, 280 µl RNase A.

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The buffer set includes: 250 ml A1  250 ml A2  300 ml A3  900 ul RNase A Good for 24 Maxipreps, 48 Midiprep-II preps and 96 Midipreps

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Good for plasmid midiprep and maxiprep kits. Not for plasmid minipreps.

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