This product is designed to remove most of the organic compounds and chlorine from tap water to protect RO membranes.
Purification Technology
Activated Carbon
Suitable for the following systems
Prefiltration Kit (2-stage)
Prefiltration Kit (3-stage)
The virus purification kit is designed for fast and efficient purification of recombinant Adenoviruses from transfected cell culture supernatant of 6-8 T75 flasks. Up to 4x1012 viral particles could be purified. The elution buffer could be exchanged with any proper buffer and virus could be concentrated by using de-salting column, which is included.
Kit's Contents
SKU
AV02-00
AV02-01
AV02-02
Store @
Preps
1
4
10
-
AV Maxi Columns
1
2
5
4°C
Centrifugal Filter*
1
2
5
RT
Press-On Cap
1
4
10
RT
50 ml Collection Tube
1
4
10
RT
10x AV Wash Buffer
10 ml
40 ml
80 ml
RT
2x AV Elution
10 ml
40 ml
80 ml
RT
Regeneration Buffer
10 ml
30 ml
50 ml
RT
The virus purification kit is designed for fast and efficient purification of recombinant Adenoviruses from transfected cell culture supernatant of 6-8 T75 flasks. Up to 4x1012 viral particles could be purified. The elution buffer could be exchanged with any proper buffer and virus could be concentrated by using de-salting column, which is included.
Kit's Contents
SKU
AV02-00
AV02-01
AV02-02
Store @
Preps
2
4
10
-
AV Maxi Columns
1
2
5
4°C
Centrifugal Filter*
1
2
5
RT
Press-On Cap
1
4
10
RT
50 ml Collection Tube
1
4
10
RT
10x AV Wash Buffer
10 ml
40 ml
80 ml
RT
2x AV Elution
10 ml
40 ml
80 ml
RT
Regeneration Buffer
10 ml
30 ml
50 ml
RT
The virus purification kit is designed for fast and efficient purification of recombinant Adenoviruses from transfected cell culture supernatant of 6-8 T75 flasks. Up to 4x1012 viral particles could be purified. The elution buffer could be exchanged with any proper buffer and virus could be concentrated by using de-salting column, which is included.
Kit's Contents
SKU
AV02-00
AV02-01
AV02-02
Store @
Preps
1
4
10
-
AV Maxi Columns
1
2
5
4°C
Centrifugal Filter*
1
2
5
RT
Press-On Cap
1
4
10
RT
50 ml Collection Tube
1
4
10
RT
10x AV Wash Buffer
10 ml
40 ml
80 ml
RT
2x AV Elution
10 ml
40 ml
80 ml
RT
Regeneration Buffer
10 ml
30 ml
50 ml
RT
The virus purification kit is designed for fast and efficient purification of recombinant Adenoviruses from transfected cell culture supernatant of 1-2 T75 flasks. Up to 1x1012 viral particles could be purified. The elution buffer could be exchanged with any proper buffer and virus could be concentrated by using de-salting column (centrifugation column), which is included.
Important: each purification column is used for 2 preps with the same virus.
Kit Contents
SKU
AV01-00
AV01-01
AV01-02
Store @
Preps
2
10
20
-
Purification Columns
1
5
10
4°C
Press-On Cap
1
5
10
RT
Desalting Columns
1
5
10
4°C
15 ml Collection Tube
2
10
20
RT
10x Wash Buffer
5 ml
30 ml
60 ml
RT
2x Elution Buffe
5 ml
30 ml
60 ml
RT
Regeneration Buffer
5 ml
30 ml
60 ml
RT
The virus purification kit is designed for fast and efficient purification of recombinant Adenoviruses from transfected cell culture supernatant of 1-2 T75 flasks. Up to 1x1012 viral particles could be purified. The elution buffer could be exchanged with any proper buffer and virus could be concentrated by using de-salting column (centrifugation column), which is included.
Important: each purification column is used for 2 preps with the same virus.
Kit Contents
SKU
AV01-00
AV01-01
AV01-02
Store @
Preps
2
10
20
-
Purification Columns
1
5
10
4°C
Press-On Cap
1
5
10
RT
Desalting Columns
1
5
10
4°C
15 ml Collection Tube
2
10
20
RT
10x Wash Buffer
5 ml
30 ml
60 ml
RT
2x Elution Buffe
5 ml
30 ml
60 ml
RT
Regeneration Buffer
5 ml
30 ml
60 ml
RT
The virus purification kit is designed for fast and efficient purification of recombinant Adenoviruses from transfected cell culture supernatant of 1-2 T75 flasks. Up to 1x1012 viral particles could be purified. The elution buffer could be exchanged with any proper buffer and virus could be concentrated by using de-salting column (centrifugation column), which is included.
Important: each purification column is used for 2 preps with the same virus.
Kit Contents
SKU
AV01-00
AV01-01
AV01-02
Store @
Preps
2
10
20
-
Purification Columns
1
5
10
4°C
Press-On Cap
1
5
10
RT
Desalting Columns
1
5
10
4°C
15 ml Collection Tube
2
10
20
RT
10x Wash Buffer
5 ml
30 ml
60 ml
RT
2x Elution Buffe
5 ml
30 ml
60 ml
RT
Regeneration Buffer
5 ml
30 ml
60 ml
RT
Working without pump oil.
Adjustable suction flow rate: 0-10 L/min.
Universal voltage 110V~220V.
Overload protection, over temperature protection, short circuit protection.
Can work without stopping for a long time, over 24 hours.
Technical Data
Pumping speed
10L/min
Negative Pressure
0.075 Mpa
Noise Level
<40 dB
Motor power
20W
Diaphragm
NBR
Overload protection
Yes
Over temperature protection
Yes
Short circuit protection
Yes
Dimension [D×W×H]:
192x100x135 mm
Weight
1.6 kg
Grade: Electrophoresis grade (DNase/RNase free)
Synonym(s): 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE
CAS Number: 9012-36-6
EC Number: 232-731-8
Application: Ideal for separating nucleic acids of a wide range of sizes
Storage Conditions: Cool, dry place
Analytical Specifications
Appearance
White Powder
Impurities
≤7% water
EEO
≤0.12
Gel Strength of 1% (w/v) gel
>1200 g /cm²
Melting Point of 1.5% (w/v) gel
87-89°C
Transition Temperature of 1.5% (w/v) gel
35-37°C
Grade: Electrophoresis grade (DNase/RNase free)
Synonym(s): 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE
CAS Number: 9012-36-6
EC Number: 232-731-8
Application: Ideal for separating nucleic acids of a wide range of sizes
Storage Conditions: Cool, dry place
Analytical Specifications
Appearance
White Powder
Impurities
≤7% water
EEO
≤0.12
Gel Strength of 1% (w/v) gel
>1200 g /cm²
Melting Point of 1.5% (w/v) gel
87-89°C
Transition Temperature of 1.5% (w/v) gel
35-37°C
Safety Data Sheet
MSDS
3 μl or 5 μl per loading for clear visualization during electrophoresison 15-well or 10-well mini-gel, respectively.
1.5~2.5 μl per well for general Western transferring.
Apply more for thicker (> 1.5 mm) or larger gel.
The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, or add reducing agent before loading.
Description: The All Blue Prestained Protein Ladder I is a protein standard with 10 pre-stained proteins covering a wide range molecular weightsfor 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore and two reference bands ( 25 kDa and 72 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The All Blue Prestained Protein Ladder I is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.
Contents: Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM 2-mercaptoethanol, 3.6M Urea, and 15 % (v/v) Glycerol).
Quality Control: Under suggested conditions, All Blue Prestained Protein Ladder I resolves10 major bands in polyacrylamide gel with appropriate buffers andafterWestern blotting to nitrocellulose membrane.
Storage:
Stable at 25°C for 2 weeks
Stable at 4°C for 3 months
Stable at -20°C for 24 months
The One-for-All (0.1-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct loading on gel. The DNA Ladder is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easy referencing. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 10,000 bp
Concentration
86 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light Room temperature ≥ 6 months 4°C ≥ 12 months -20°C ≥ 36 months