Features: High clarity medical grade polystyrene material High performance with 5 growth surfaces Growth area: 870cm² TC treatment for better cell attachment Sterilized by E-Beam, non-pyrogenic Vent cap Adding Media and Preparing Cell Suspension: Mix cell suspension with medium: Prepare cell suspension of required concentration in a container and mix. Recommended volume is about 30- 50 mL per layer. Add the mixed liquid into the Multi-layer Flask slowly with serological pipettes. To avoid foams and bubbles, allow liquid stream to flow along the slope of the Multi-layer Flask. (Save a little liquid in pipette for each dosing) Tip: A 10 mL pipette allows media to be dispensed at the bottom of the vessel. A 25 mL pipette allows media to be dispensed just past the NEST Logo. Hold the Multi-layer Flask upright with the Logo facing you and tilt clockwise to a 45° angle on a flat work surface to partition the liquid into each layer. While holding the Multi-layer Flask at a 45° angle, gently lay it flat onto the work surface with logo facing up. After placing the Multi-layer Flask flat on a work surface, gently rock back and forth and side-to-side to distribute cells evenly onto culture surfaces. Repeat Step 3 to put the flask quickly and slightly into the incubator. Then, lay it flat as shown in Step 4 . Media Removal Aspirating method: To aspirate or remove media, tilt Multi-layer Flask, with the NEST Logo facing you, counter-clock wise to a 45° angle while inverting the Multi-Flask toward you. Then, tilt Multi-layer Flask to the right, continuing to aspirate all residual media. Pouring method: With Logo facing you, pour spent media from Multi-layer Flask Cell Harvesting Wash with buffer once and add dissociating reagent (≥5 mL per layer). Then, follow Steps 3-4 to distribute to dissociating reagent to each layer. Neutralize with inactivating solution and mix following Steps 3-4. Gently swirl to dislodge cells completely. Follow “Aspirating Method” protocol and collect cell suspension using a NEST 10mL serological pipet. Follow “Pouring Method”. Pour detached cell suspension into a NEST conical tube. 13. Rinse with additional wash buffer as needed.

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Features Brand: NEST® High clarity polystyrene Thin wall design to reduce edge effect No vacuum plasma TC treatment, good for suspension cell culture Nonreversible lids with condensation to prevent cross-contamination Easy peelable, individually wrapped package Sterilized by E-Beam Non-pyrogenic SKU Type (well) Bottom TC Treatment Recommended Medium Volume Cell Growth Area (cm²) 703003 6 Flat Yes 3 9.5 703011 6 Flat No 3 9.5 712003 12 Flat Yes 2 3.6 712011 12 Flat No 2 3.6 702003 24 Flat Yes 1 1.9 702011 24 Flat No 1 1.9 748003 48 Flat Yes 0.5 0.95 748011 48 Flat No 0.5 0.95 701002 96 Flat Yes 0.1-0.2 0.32 701003 96 Flat Yes 0.1-0.2 0.32 701011 96 Flat No 0.1-0.2 0.32 701101 96 U Yes 0.1-0.2 0.32 701111 96 U No 0.1-0.2 0.32 701201 96 V Yes 0.1-0.2 0.32 701301 96 Flat Yes 0.1-0.2 0.32

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Features Brand: NEST® High clarity polystyrene Thin wall design to reduce edge effect Vacuum plasma TC treatment Nonreversible lids with condensation to prevent cross-contamination Easy peelable, individually wrapped package Sterilized by E-Beam Non-pyrogenic SKU Type (well) Bottom TC Treatment Recommended Medium Volume Cell Growth Area (cm²) 703003 6 Flat Yes 3 9.5 703011 6 Flat No 3 9.5 712003 12 Flat Yes 2 3.6 712011 12 Flat No 2 3.6 702003 24 Flat Yes 1 1.9 702011 24 Flat No 1 1.9 748003 48 Flat Yes 0.5 0.95 748011 48 Flat No 0.5 0.95 701002 96 Flat Yes 0.1-0.2 0.32 701003 96 Flat Yes 0.1-0.2 0.32 701011 96 Flat No 0.1-0.2 0.32 701101 96 U Yes 0.1-0.2 0.32 701111 96 U No 0.1-0.2 0.32 701201 96 V Yes 0.1-0.2 0.32 701301 96 Flat Yes 0.1-0.2 0.32  

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Features Brand: NEST® High clarity polystyrene Flat transparent surface for distortion-free observation Vacuum plasma TC treatment, excellent cell adherence PA/PE packaging Stackable for easy storage and handling Sterilized by E-Beam SKU Type Height (mm) Cell Growth Area (cm²) Per Bag Total 706001 35mm 9.0 9 20 500 705001 60mm 12.0 21 20 500 704001 100mm 17.7 55 20 300 704004 100mm 17.7 55 5 300 704201 100mm 17.7 55 20 300 715001 150mm 23.1 152 5 100

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Features High clarity polystyrene Flat transparent surface Numeric mark on the bottom for easy identification PA/PE packaging Vent for gas exchange Stackable for easy storage and handling Sterilized by E-Beam, SAL=10-6 Non-pyrogenic 60x15mm

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Features Brand: NEST® High clarity polystyrene Vacuum plasma TC treatment for better cell attachment Non-treated flask for suspension cells is available Sterilized by E-Beam Non-pyrogenic SKU Cell Growth Area (cm²) Volume (ml) Cap Type TC Treatment Per Bag Total 707001 25 50 Plug Seal Yes 10 200 708001 75 250 Plug Seal Yes 5 100 709001 175 650 Plug Seal Yes 5 40 721001 225 950 Plug Seal Yes 5 25 707003 25 50 Vent Yes 10 200 707013 25 50 Vent No 10 200 708003 75 250 Vent Yes 5 100 708013 75 250 Vent No 5 100 709003 175 650 Vent Yes 5 40 721003 225 950 Vent Yes 5 25

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Features Brand: NEST® High clarity polystyrene No vacuum plasma TC treatment, good for suspension cell culture Vent cap with 0.22 um hydrophobic filters for gas exchange and preventing microorganisms Sterilized by E-Beam Non-pyrogenic SKU Cell Growth Area (cm²) Volume (ml) Cap Type TC Treatment Per Bag Total 707001 25 50 Plug Seal Yes 10 200 708001 75 250 Plug Seal Yes 5 100 709001 175 650 Plug Seal Yes 5 40 721001 225 950 Plug Seal Yes 5 25 707003 25 50 Vent Yes 10 200 707013 25 50 Vent No 10 200 708003 75 250 Vent Yes 5 100 708013 75 250 Vent No 5 100 709003 175 650 Vent Yes 5 40 721003 225 950 Vent Yes 5 25

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Features Brand: NEST® High clarity polystyrene Vacuum plasma TC treatment for better cell attachment Non-treated flask for suspension cells is available Vent cap with 0.22 um hydrophobic filters for gas exchange and preventing microorganisms Sterilized by E-Beam Non-pyrogenic SKU Cell Growth Area (cm²) Volume (ml) Cap Type TC Treatment Per Bag Total 707001 25 50 Plug Seal Yes 10 200 708001 75 250 Plug Seal Yes 5 100 709001 175 650 Plug Seal Yes 5 40 721001 225 950 Plug Seal Yes 5 25 707003 25 50 Vent Yes 10 200 707013 25 50 Vent No 10 200 708003 75 250 Vent Yes 5 100 708013 75 250 Vent No 5 100 709003 175 650 Vent Yes 5 40 721003 225 950 Vent Yes 5 25

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Features Brand: NEST® High clarity polystyrene Thin wall design to reduce edge effect No vacuum plasma TC treatment, good for suspension cell culture Nonreversible lids with condensation to prevent cross-contamination Easy peelable, individually wrapped package Sterilized by E-Beam Non-pyrogenic SKU Type (well) Bottom TC Treatment Recommended Medium Volume Cell Growth Area (cm²) 703003 6 Flat Yes 3 9.5 703011 6 Flat No 3 9.5 712003 12 Flat Yes 2 3.6 712011 12 Flat No 2 3.6 702003 24 Flat Yes 1 1.9 702011 24 Flat No 1 1.9 748003 48 Flat Yes 0.5 0.95 748011 48 Flat No 0.5 0.95 701002 96 Flat Yes 0.1-0.2 0.32 701003 96 Flat Yes 0.1-0.2 0.32 701011 96 Flat No 0.1-0.2 0.32 701101 96 U Yes 0.1-0.2 0.32 701111 96 U No 0.1-0.2 0.32 701201 96 V Yes 0.1-0.2 0.32 701301 96 Flat Yes 0.1-0.2 0.32

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Features Brand: NEST® High clarity polystyrene Thin wall design to reduce edge effect Vacuum plasma TC treatment Nonreversible lids with condensation to prevent cross-contamination Easy peelable, individually wrapped package Sterilized by E-Beam Non-pyrogenic SKU Type (well) Bottom TC Treatment Recommended Medium Volume Cell Growth Area (cm²) 703003 6 Flat Yes 3 9.5 703011 6 Flat No 3 9.5 712003 12 Flat Yes 2 3.6 712011 12 Flat No 2 3.6 702003 24 Flat Yes 1 1.9 702011 24 Flat No 1 1.9 748003 48 Flat Yes 0.5 0.95 748011 48 Flat No 0.5 0.95 701002 96 Flat Yes 0.1-0.2 0.32 701003 96 Flat Yes 0.1-0.2 0.32 701011 96 Flat No 0.1-0.2 0.32 701101 96 U Yes 0.1-0.2 0.32 701111 96 U No 0.1-0.2 0.32 701201 96 V Yes 0.1-0.2 0.32 701301 96 Flat Yes 0.1-0.2 0.32  

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Features Brand: NEST® High clarity polystyrene Thin wall design to reduce edge effect Vacuum plasma TC treatment Nonreversible lids with condensation to prevent cross-contamination Easy peelable, individually wrapped package Sterilized by E-Beam Non-pyrogenic SKU Type (well) Bottom TC Treatment Recommended Medium Volume Cell Growth Area (cm²) 703003 6 Flat Yes 3 9.5 703011 6 Flat No 3 9.5 712003 12 Flat Yes 2 3.6 712011 12 Flat No 2 3.6 702003 24 Flat Yes 1 1.9 702011 24 Flat No 1 1.9 748003 48 Flat Yes 0.5 0.95 748011 48 Flat No 0.5 0.95 701002 96 Flat Yes 0.1-0.2 0.32 701003 96 Flat Yes 0.1-0.2 0.32 701011 96 Flat No 0.1-0.2 0.32 701101 96 U Yes 0.1-0.2 0.32 701111 96 U No 0.1-0.2 0.32 701201 96 V Yes 0.1-0.2 0.32 701301 96 Flat Yes 0.1-0.2 0.32  

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Features Brand: NEST® High clarity polystyrene Thin wall design to reduce edge effect Vacuum plasma TC treatment Nonreversible lids with condensation to prevent cross-contamination Easy peelable, individually wrapped package Sterilized by E-Beam Non-pyrogenic SKU Type (well) Bottom TC Treatment Recommended Medium Volume Cell Growth Area (cm²) 703003 6 Flat Yes 3 9.5 703011 6 Flat No 3 9.5 712003 12 Flat Yes 2 3.6 712011 12 Flat No 2 3.6 702003 24 Flat Yes 1 1.9 702011 24 Flat No 1 1.9 748003 48 Flat Yes 0.5 0.95 748011 48 Flat No 0.5 0.95 701002 96 Flat Yes 0.1-0.2 0.32 701003 96 Flat Yes 0.1-0.2 0.32 701011 96 Flat No 0.1-0.2 0.32 701101 96 U Yes 0.1-0.2 0.32 701111 96 U No 0.1-0.2 0.32 701201 96 V Yes 0.1-0.2 0.32 701301 96 Flat Yes 0.1-0.2 0.32  

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