Products
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DNase I, RNase-Free (100 mg)
SKU: DNI01-100MGSpecific Activity: ≥ 2000 Kunitz units/mg Form: Lyophilized Powder Storage: 4°C DNase Ⅰ (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids. Feature Recombinant enzyme Purified from non-animal host with a lower level of intrinsic RNases. Applications Degradation of DNA template in transcription reactions Removal of contaminating genomic DNA from RNA samples DNase I footprinting Nick Translation Quality Control The absence of ribonucleases is confirmed by appropriate quality test. Functionally tested for digestion of template DNA after in vitro transcription Source: A E. Coli strain that carries an MBP fusion clone of Bovine Pancreatic DNase I. Molecular Weight: 29 kDa monomer Definition of Activity Unit One unit of the enzyme completely degrades 1 μg of plasmid DNA in 10 min at 37°C. Enzyme activity is assayed in the following mixture: 40 mM Tris-HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2, 1 μg of pUC19 DNA. Inhibition and Inactivation Inhibitors: metal chelators, transition metals (e.g., Zn) in millimolar concentrations, SDS (even at concentrations less than 0.1%), reducing agents (DTT and beta-mercaptoethanol), ionic strength above 50-100 mM. Inactivated by heating at 65°C for 10 min in the presence of EGTA or EDTA (use at least 1 mol of EGTA/EDTA per 1 mol of Mn2+/Mg2+). Note: DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not Vortex. Preparation InstructionsDNase I is soluble in 0.15 M NaCl at 5 mg/ml. Solutions of DNase I at 5 mg/ml in 0.15 M NaCl may lose 10% of its activity when stored for a week in aliquots at -20°C. The same solutions stored in aliquots at 2-8°C can lose 20% activity. Alternatively, solutions at a minimum concentration of 1 mg protein/ml, with 5 mM calcium chloride and stored at -20°C, may retain 90% activity for at least a year. Solutions containing 0.1 mg protein/ml are considerably less stable, and may require gelatin as stabilizer. For applications unaffected by glycerol, two other storage buffers are options, as these formulations do not freeze at -20°C. 20 mM sodium acetate (pH 6.5), containing 5 mM CaCl2 and 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 50% (v/v) glycerol, with DNase I at 5 mg/ml. 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 10 mM MgCl2, 1 mM DTT, 50% (v/v) glycerol, with DNase I at 2 mg/ml.
$58.80
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dNTP (100mM), >99% HPLC, 1ml
SKU: NTA01-1ML>99.0%, HPLC Applications For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing and DNA labeling. Quality Control Functionally tested in PCR with Taq and Pfu DNA Polymerases. Greater than 99% purity of each component confirmed by HPLC. Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease and phosphatase activities. Storage Long Term (Infrequent use; 1-2 times per month): -70°C Daily/Weekly use: -20°C
$52.00 - $70.00
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dNTP Mix (100mM dNTP)
SKU: NT03-10100mM dNTP Mix is a premixed solution containing 25mM of each dATP, dCTP, dGTP and dTTP. dNTP Mixes are ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications. Applications For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing and DNA labeling. Quality Control Functionally tested in PCR with Taq and Pfu DNA Polymerases. Greater than 99% purity of each component confirmed by HPLC. Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease and phosphatase activities. Storage Long Term (Infrequent use; 1-2 times per month): -70°C Daily/Weekly use: -20°C
$400.00 - $1,500.00
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dNTP Mix (10mM per dNTP)
SKU: NT01-0110mM dNTP Mix is a premixed solution containing 10mM of each dATP, dCTP, dGTP and dTTP. dNTP Mixes are ready-to-use solutions designed to save time and to provide higher reproducibility in PCR and other applications. Applications For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing and DNA labeling. Quality Control Functionally tested in PCR with Taq and Pfu DNA Polymerases. Greater than 99% purity of each component confirmed by HPLC. Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease and phosphatase activities. Storage Long Term (Infrequent use; 1-2 times per month): -70°C Daily/Weekly use: -20°C ***** This is a cold item. You are requires to select Overnight shipping for purchase this item! *****
$35.30 - $123.50
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dNTP Set (100 mM per dNTP)
SKU: NT02-01dNTP set consits of 100 mM aqueous solutions of each of dATP, dCTP, dGTP and dTTP at pH 7.0, each in a separate vial. dNTP set offers maximum flexibility in preparation of reaction mixes for different applications. Applications For use in all molecular biology applications, including PCR, long PCR, real-time PCR, high fidelity PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing and DNA labeling. Quality Control Functionally tested in PCR with Taq and Pfu DNA Polymerases. Greater than 99% purity of each component confirmed by HPLC. Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease and phosphatase activities. Storage Long Term (Infrequent use; 1-2 times per month): -70°C Daily/Weekly use: -20°C ***** This is a cold item. You are requires to select Overnight shipping for purchase this item! *****
$64.70 - $5,500.00
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dPette+ Multi-functional 8-Channel Electronic Pipette (0.5-10ul)
SKU: EPIPET8C-10The DLAB Multi-channel Electric Pipette is an ideal solution for laboratories that require fast, accurate, and efficient pipetting of multiple samples. Its ergonomic design, precise volume control, and programmable features make it an excellent choice for a range of liquid transfer applications. Precision and Parallelism: The DLAB pipette features a high-precision step motor that ensures accuracy and parallelism during pipetting, minimizing errors and improving data repeatability. Ergonomic Design: Its lightweight streamlined design is ergonomically engineered to reduce fatigue and increase efficiency during extended use, ultimately enhancing the unit time of liquid transfer and experimental data reproducibility. Rechargeable Battery: The DLAB pipette can be recharged via a desktop or power cord, providing users with the flexibility to choose the best charging method for their laboratory. Programmable Volume Range: The DLAB pipette offers four programmable volume ranges: 0.5-10 µl, 5-50 µl, 15-300 µl, and 30-300 µl, allowing for a wide range of liquid transfer needs. User-Friendly Interface: Its large LCD screen provides a user-friendly interface that simulates manual pipetting operations and displays critical parameters, making it easy to use and set different functions. Dual Rotary Knobs: The DLAB pipette features dual rotary knobs that enable all functions to be performed seamlessly, providing users with a simple and intuitive operation. Adjustable Speed: Users can adjust the aspiration and dispensing speeds to suit their specific requirements, enhancing the device's versatility. Functional Modes: The DLAB pipette has four functional modes: liquid aspirating and dispensing, continuous mixing, continuous dispensing, and diluting, providing a variety of options to accommodate different pipetting tasks. Autoclavable Components: The DLAB pipette features autoclavable components that allow users to sterilize the device at high temperatures and pressures. Automatic Calibration: The DLAB pipette can be calibrated automatically by connecting it to a USB-enabled balance, eliminating the need to send the device for third-party calibration. Product Video Volume Range (μl) Increment (μl) Test Volume (μl) Systematic error (±%) Systematic error(±μl) Random error (±%) Random error(±μl) 0.5-10 0.01 10 0.20 2.00 0.10 1.00 5 0.20 4.00 0.10 2.00 1 0.08 8.00 0.05 5.00
$935.00
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dPette+ Multi-functional 8-Channel Electronic Pipette (10-100ul)
SKU: EPIPET8C-100The DLAB Multi-channel Electric Pipette is an ideal solution for laboratories that require fast, accurate, and efficient pipetting of multiple samples. Its ergonomic design, precise volume control, and programmable features make it an excellent choice for a range of liquid transfer applications. Precision and Parallelism: The DLAB pipette features a high-precision step motor that ensures accuracy and parallelism during pipetting, minimizing errors and improving data repeatability. Ergonomic Design: Its lightweight streamlined design is ergonomically engineered to reduce fatigue and increase efficiency during extended use, ultimately enhancing the unit time of liquid transfer and experimental data reproducibility. Rechargeable Battery: The DLAB pipette can be recharged via a desktop or power cord, providing users with the flexibility to choose the best charging method for their laboratory. Programmable Volume Range: The DLAB pipette offers four programmable volume ranges: 0.5-10 µl, 5-50 µl, 15-300 µl, and 30-300 µl, allowing for a wide range of liquid transfer needs. User-Friendly Interface: Its large LCD screen provides a user-friendly interface that simulates manual pipetting operations and displays critical parameters, making it easy to use and set different functions. Dual Rotary Knobs: The DLAB pipette features dual rotary knobs that enable all functions to be performed seamlessly, providing users with a simple and intuitive operation. Adjustable Speed: Users can adjust the aspiration and dispensing speeds to suit their specific requirements, enhancing the device's versatility. Functional Modes: The DLAB pipette has four functional modes: liquid aspirating and dispensing, continuous mixing, continuous dispensing, and diluting, providing a variety of options to accommodate different pipetting tasks. Autoclavable Components: The DLAB pipette features autoclavable components that allow users to sterilize the device at high temperatures and pressures. Automatic Calibration: The DLAB pipette can be calibrated automatically by connecting it to a USB-enabled balance, eliminating the need to send the device for third-party calibration. Product Video Volume Range (μl) Increment (μl) Test Volume (μl) Systematic error (±%) Systematic error(±μl) Random error (±%) Random error(±μl) 10-100 0.1 100 0.80 0.80 0.30 0.30 50 0.50 1.00 0.40 0.80 10 0.30 3.00 0.20 2.00
$935.00
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dPette+ Multi-functional 8-Channel Electronic Pipette (15-300ul)
SKU: EPIPET8C-300The DLAB Multi-channel Electric Pipette is an ideal solution for laboratories that require fast, accurate, and efficient pipetting of multiple samples. Its ergonomic design, precise volume control, and programmable features make it an excellent choice for a range of liquid transfer applications. Precision and Parallelism: The DLAB pipette features a high-precision step motor that ensures accuracy and parallelism during pipetting, minimizing errors and improving data repeatability. Ergonomic Design: Its lightweight streamlined design is ergonomically engineered to reduce fatigue and increase efficiency during extended use, ultimately enhancing the unit time of liquid transfer and experimental data reproducibility. Rechargeable Battery: The DLAB pipette can be recharged via a desktop or power cord, providing users with the flexibility to choose the best charging method for their laboratory. Programmable Volume Range: The DLAB pipette offers four programmable volume ranges: 0.5-10 µl, 5-50 µl, 15-300 µl, and 30-300 µl, allowing for a wide range of liquid transfer needs. User-Friendly Interface: Its large LCD screen provides a user-friendly interface that simulates manual pipetting operations and displays critical parameters, making it easy to use and set different functions. Dual Rotary Knobs: The DLAB pipette features dual rotary knobs that enable all functions to be performed seamlessly, providing users with a simple and intuitive operation. Adjustable Speed: Users can adjust the aspiration and dispensing speeds to suit their specific requirements, enhancing the device's versatility. Functional Modes: The DLAB pipette has four functional modes: liquid aspirating and dispensing, continuous mixing, continuous dispensing, and diluting, providing a variety of options to accommodate different pipetting tasks. Autoclavable Components: The DLAB pipette features autoclavable components that allow users to sterilize the device at high temperatures and pressures. Automatic Calibration: The DLAB pipette can be calibrated automatically by connecting it to a USB-enabled balance, eliminating the need to send the device for third-party calibration. Product Video Volume Range (μl) Increment (μl) Test Volume (μl) Systematic error (±%) Systematic error(±μl) Random error (±%) Random error(±μl) 15-300 1 300 1.80 0.60 0.90 0.30 150 1.50 1.00 0.75 0.50 15 0.60 4.00 0.15 1.00
$935.00
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dPette+ Multi-functional Electronic Pipette (0.5-10ul)
SKU: EPIPET10Motor driven and digitally controlled Functions: Pipetting, Mixing, Stepper (multiple dispensing) and Diluting Intuitive menu interface setting display 2 buttons for all operational settings Li-ion rechargeable battery enables longer operation time Adjustable speed for aspiration and dispensing Minimal force for pipetting operation High performance ensuring accuracy & repeatability Self-calibration applicable Product Video Volume Range (μl) Increment (μl) Test Volume (μl) Systematic error (±%) Systematic error(±μl) Random error (±%) Random error(±μl) 0.5-10 0.01 10 0.10 1.00 0.05 0.50 5 0.10 2.00 0.10 2.00 1 0.80 5.00 0.03 3.00
$552.00
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dPette+ Multi-functional Electronic Pipette (100-1000ul)
SKU: EPIPET1000Motor driven and digitally controlled Functions: Pipetting, Mixing, Stepper (multiple dispensing) and Diluting Intuitive menu interface setting display 2 buttons for all operational settings Li-ion rechargeable battery enables longer operation time Adjustable speed for aspiration and dispensing Minimal force for pipetting operation High performance ensuring accuracy & repeatability Self-calibration applicable Product Video Volume Range (μl) Increment (μl) Test Volume (μl) Systematic error (±%) Systematic error(±μl) Random error (±%) Random error(±μl) 100-1000 5 1000 6.00 0.60 2.00 0.20 5 5.00 1.00 1.00 0.20 1 3.00 3.00 0.60 0.60
$552.00
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dPette+ Multi-functional Electronic Pipette (30-300ul)
SKU: EPIPET300Motor driven and digitally controlled Functions: Pipetting, Mixing, Stepper (multiple dispensing) and Diluting Intuitive menu interface setting display 2 buttons for all operational settings Li-ion rechargeable battery enables longer operation time Adjustable speed for aspiration and dispensing Minimal force for pipetting operation High performance ensuring accuracy & repeatability Self-calibration applicable Product Video Volume Range (μl) Increment (μl) Test Volume (μl) Systematic error (±%) Systematic error(±μl) Random error (±%) Random error(±μl) 30-300 1 300 1.80 0.60 0.60 0.20 100 1.00 1.00 0.40 0.40 30 1.20 4.00 0.21 0.70
$552.00
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dPette+ Multi-functional Electronic Pipette (5-50ul)
SKU: EPIPET50Motor driven and digitally controlled Functions: Pipetting, Mixing, Stepper (multiple dispensing) and Diluting Intuitive menu interface setting display 2 buttons for all operational settings Li-ion rechargeable battery enables longer operation time Adjustable speed for aspiration and dispensing Minimal force for pipetting operation High performance ensuring accuracy & repeatability Self-calibration applicable Product Video Volume Range (μl) Increment (μl) Test Volume (μl) Systematic error (±%) Systematic error(±μl) Random error (±%) Random error(±μl) 5-50 0.1 50 0.40 0.80 0.15 0.3 25 0.25 1.00 0.25 1.00 5 0.2 4.00 0.125 2.50
$552.00