Tris-Glycine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye. To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results. Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis. Composition: 125 mM Tris HCl, 4% SDS, 20% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).   MSDS

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Tris-Glycine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using Tris-Glycine gels. It has a pH of 6.8 and contains bromophenol blue as a tracking dye. To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2–5 minutes for optimal results. Note: Heating samples at 100°C in SDS-containing buffers results in proteolysis. Composition: 125 mM Tris HCl, 4% SDS, 20% Glycerol, 0.01% Bromophenol blue (Non-reduced). Add ß-mercaptoethanol (8% V/V) before use (Reduced).   MSDS

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Features The kit is designed for multiplex qPCR with TaqMan probes. The kit uses Super Taq-Probe DNA polymerase specially engineered for TaqMan probes, which increases 5’ to 3’ exonuclease efficiency and produces S-shaped curve. Up to four pairs of gene-specific primers can be applied in one reaction. The concentrations of the primers and probes are variable depending on specific assays and thermocycling protocols (Table 1).  The preferable PCR product size is ≤150bp. The kit has three formulations of No ROX, Low ROX or High ROX concentrations for your choice (see Table 2). Intended Use The 2x Multiplex qPCR Master Mix is used for real-time qualitative and quantitative multiplex qPCR with TaqMan probes for up to four targets.  The master mix is a premixed, 2X concentrated solution that has all the components except for gene-specific primers, probes and templates. Transportation and storage The kit can be transported at below 4°C for up to 3 days.  The kit should be kept stable in the dark at -20°C for ≤24 months with ≤10 times of freeze-thaw cycles. The kit can be stored at 4°C for a week.  Setup Reaction and Thermocycling  Thaw 2x Master mix and other reaction components at room temperature, mix each component, centrifuge and then place on ice.    Set up reactions (Table 1).  Seal tubes with flat, optically transparent caps or seal plate with optically transparent film. Mix and then briefly centrifuge the tubes or plate. Program PCR instrument with indicated thermo-cycling protocol. Load PCR tubes or plate and start to run. Perform data analysis according to the PCR instrument instructions. Table 1. Setting up a 20μl or 10μl Reaction Component Volume per 20μl Volume per 10μl Final Concentration 2x Mastermix 10μL 5μL 1X Primersa Variable Variable Each 150-400nM TaqMan probesb Variable Variable Each 150-250nM DNA Templatec Variable Variable ≤500ng human genomic DNA/20μL H2O To 20μL To 10μL Note: Each primer’s Tm should be designed ≥60°C, preferably between 62°C to 65°C, using primer3 software for high efficiency and specificity. Each probe’s Tm should be 8-10°C higher than the primer’s Tm, preferably between 70-75°C. DNA templates should be extracted by a qualified silica-based kit and eluted with low EDTA TE buffer (10mM Tris-HCl, 0.1mM EDTA, pH 8.0-8.3). Table 2. Standard Thermocycling Protocol Stage Temperature Period Number of Cycles I 95°C 2 min 1 II 95°C 10 sec 35-40 II 60°C, signal acquisition 60 sec 35-40 Table 3. Three-Step Thermocycling Protocol Stage Temperature Period Number of Cycles I 95°C 1 min 1 II 95°C 10 sec 35-40 II 60°C 30 sec 35-40 II 68-72°C, signal acquisition 30 sec 35-40 Notes: The three-step thermocycling protocol in Table 4 increases overall polymerase activity by 50%, a more effective protocol than Table 3. The primer concentration used in Tables 3 and 4 is typically 0.15-0.2uM. Precautions If you order a “No ROX” master mix but you have an Applied Biosystems or Thermo Fisher instrument, please turn off ROX passive reference dye button when setup assays. Table 4. Compatible Instruments qPCR Instrument ROX required by instrument Passive dye setup Bio-Rad® iQ™5, CFX96, CFX384, Opticon Roche Lightcycler®Qiagen Rotor-Gene™Eppendorf Mastercycler®Cepheid® SmartCycler® Not recommended Not necessary Applied Biosystems® 7500, 7500 Fast, QuantStudio™, ViiA7™, Agilent Mx™ Low ROX(50nM final concentration) Turn on ROX passive reference dye  Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne™, StepOnePlus™ High ROX(500nM final concentration) Turn on ROX passive reference dye 

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SYBR Green I-based qPCR reagent: It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg2+ and ROX calibration dye. It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes. The chemically modified PowerPCR™ Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes. The unique PCR buffer components and the Hot start enzyme ensure PCR specificity and sensitivity. The ROX is a dye molecule that can be used to normalize the well-to-well fluorescent fluctuations and can be applied to all qPCR instruments using ROX as calibration dye. It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or low copy template. Suitable Real-Time PCR detection systems: ABI Prism7000/7300/7700/7900 ABI Step One/StepOne Plus Eppendorf systems need high ROX signals

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SYBR Green I-based qPCR reagent: It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg2+ and ROX calibration dye. It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes. The chemically modified PowerPCR™ Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes. The unique PCR buffer components and the Hot start enzyme ensure PCR specificity and sensitivity. The ROX is a dye molecule that can be used to normalize the well-to-well fluorescent fluctuations and can be applied to all qPCR instruments using ROX as calibration dye. It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or low copy template. Suitable Real Time PCR Detection Systems ABI Prism 7500/7500 Fast Strategene MX3000/MX3005P Corbett Rotor Gene 3000 Systems need low ROX signals

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SYBR Green I-based qPCR reagent: It contains PowerPCR™ Hot Start Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes and Mg2+. It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to double-stranded (ds) DNA without using sequence-specific probes. The chemically modified PowerPCR™ Hot Start Taq DNA polymerase is inactive at room temperature which effectively avoids the nonspecific amplification caused by primer dimers or the nonspecific binding of prime and template. The enzyme should be activated by incubation at 95°C for 10 minutes. The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity. It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or the low copy template.

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The paper tapes are ideal for general laboratory use, and is excellent for applications in refrigeration. Sticks to virtually any surface. Can be written on with pencil, fiber-tip or ball point pen, and lab markers. Can withstand temperatures from -23 to 121°C (-10 to 250°F) and has an application temperature as low as 0°C (32°F). Vinyl-coated, soft satin-finish The adhesive backing will peel off easily and leaves no residue Rainbow packs contain blue, green, orange, pink, red, yellow, and white tape.

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30 Liter PE Tank RATK030L0 is designed to protect the quality of purified water based on the best proven technology & designs. Features:  High purity inert HDPE material. HDPE has the little branching making the tank is low extractable and has smooth surface. Opaque construction and smooth inner surface to minimize biofilm formation and algal growth. No dead volume thanks to cylindrical shape and conical bottom design. Easy in emptying and cleaning. Threaded lid for airtight closure, preventing air from entering inside the tank. A vent filter to guard the water tank against particles and microbes from atmosphere. Continuous liquid level sensor for exact measurement of water level at any point. Overflow sensor managed by control console. Convenient and worry-free. Suitable for Genie water systems: Genie G Genie U Genie E Genie R Genie PURIST (Optional) Dimensions: Length 420 mm Depth 340 mm Height 580 mm Volume 30 L

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Applications: Suitable for product packaging and storage requirements in molecular biology and cell biology, laboratory medicine, genomics, proteomics, and other areas. Product Features The raw materials, premium polypropylene (PP, cap)/polyethylene (HDPE, bottle), have excellent physical and chemical indicators and very good pressure-resistance, impact-resistance and acid and alkali resistance. PP materials can undergo autoclave sterilization at 121°C; HDPE can be stored at -80°C. A full range of choices to meet different packaging and storage requirements. Wash-free, no need for tedious pre-cleaning processing work, ready to use, greatly improves the packaging effect of users. Thickened middle packaging to ensure transportation and storage safety. Use of professional leak-proof design for bottle mouth, with no need for inner cover or inner gasket for protection, excellent sealability. 100% leak-proof to ensure safety even during air transportation; wide-mouthed design for easy access of liquid. No biotoxicity, pyrogen-free, DNase/RNase-free, manufactured in a 100-thousand grade clean plant. Sterilized using GAMMA-ray. Introduction of imported digital-controlled production equipment with high precision Fine details and comfortable touch Bottle walls are even with high gloss, with no color difference; high uniformity between different batches.

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The PP bottles are for general laboratory use, ideal for lab specimens, chemicals and buffers. Features: Excellent chemical resistance to alcohols, alkalis and acids Autoclavable Can be stored in -40°C freezer Durable Leakproof design Polypropylene caps included SKU Capacity Diameter x Height Cap Size BO3W10-10 BO3W10-100 BO3W10-1000 10 ml (0.3 fl oz) 23 x 48 mm 22 mm BO3W15-10BO3W15-100BO3W15-1000 15 ml (0.5 fl oz) 27 x 48 mm 22 mm BO3W30-10BO3W30-100BO3W30-800 30 ml (1.0 fl oz) 34 x 58 mm 31 mm BO3W60-10BO3W60-100BO3W60-400 60 ml (2.0 fl oz) 40 x 82 mm 37 mm BO3W125-10BO3W125-100BO3W125-250 125 ml (4.2 fl oz) 49 x 98 mm 43 mm BO3W250-10BO3W250-150 250 ml (8.5 fl oz) 60 x 128 mm 43 mm BO3W500-10BO3W500-120 500 ml (16.9 fl oz) 73 x 158 mm 56 mm

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Applications: Suitable for product packaging and storage requirements in molecular biology and cell biology, laboratory medicine, genomics and proteomics and other areas. Product Features The raw materials, premium polypropylene (PP)/polyethylene (HDPE), have excellent physical and chemical indicators, pressure-resistance, impact-resistance, and acid and alkali resistance. PP materials can undergo autoclave sterilization at 121°C ; HDPE can be stored at -80°C. Pre-washed/Ready-to-use: no need for tedious pre-cleaning Thickened middle packaging to ensure transportation and storage safety. Use of professional leak-proof design for bottle mouth, with no need for inner cover or inner gasket for protection, excellent sealability. 100% leak-proof to ensure safety even during air transportation; wide-mouthed design for easy access of liquid. No biotoxicity, pyrogen-free, DNAse/RNAse-free, manufactured in a 100-thousand grade clean plant. Sterilized using GAMMA-ray Using automated production equipment, the bottles have fine details and evenly glossed walls. In addition, batches are incredibly uniform By using high-grade molding and surface processing technologies, the bottles feature smooth interior and exterior surfaces that prevent the wicking effect of reagents, thereby significantly reducing sample wastage

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Applications: Suitable for product packaging and storage requirements in molecular biology and cell biology, laboratory medicine, genomics and proteomics and other areas. Product Features The raw materials, premium polypropylene (PP)/polyethylene (HDPE), have excellent physical and chemical indicators, pressure-resistance, impact-resistance, and acid and alkali resistance. PP materials can undergo autoclave sterilization at 121°C; HDPE can be stored at -80°C. Pre-washed/Ready-to-use: no need for tedious pre-cleaning Thickened middle packaging to ensure transportation and storage safety. Use of professional leak-proof design for bottle mouth, with no need for inner cover or inner gasket for protection, excellent sealability. 100% leak-proof to ensure safety even during air transportation; wide-mouthed design for easy access of liquid. No biotoxicity, pyrogen-free, DNAse/RNAse-free, manufactured in a 100-thousand grade clean plant. Sterilized using GAMMA-ray. Using automated production equipment, the bottles have fine details and evenly glossed walls. In addition, batches are incredibly uniform.  By using high-grade molding and surface processing technologies, the bottles feature smooth interior and exterior surfaces that prevent the wicking effect of reagents, thereby significantly reducing sample wastage SKU Volume (ml) Material Color Bottles per Bag Total Bottles 336101 15 HDPE Natural 20 400 337101  30 HDPE Natural 10 200 338101 60 HDPE Natural 10 200 339101 125 HDPE Natural 10 100 340101 250 HDPE Natural 10 100 341101 500 HDPE Natural 5 50

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