Current DNA electrophoresis buffers are mostly restricted to Tris-acetic acid-disodium EDTA (TAE) and Trisboric acid-disodium EDTA (TBE). The main problem of these buffers is that it generates excessive heat, limiting the voltage and speed of electrophoresis. Fast DNA Electrophoresis buffer overcomes this problem. The DNA gels can be run at high voltage (350V for 10cm gels in length) and are ready to be read in 15 minutes. The DNA extracted from the gel can be used for cloning. Usage Dilute in ddH2O to 1x Use diluted buffer to make gel and run the gel Voltage: 10-35V/cm, length of the gel Figure: Gel comprised 1.2% agarose. The gel was run fast (350 V) for 16 min in 1xFEB or 1xTBE. The run used a horizontal gel box,  700 mL total volume of buffers in the reservoirs and gels of 10 cm length. M, DNA marker (1 Kb Plus). PCR, 280-bp PCR fragment.

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Current DNA electrophoresis buffers are mostly restricted to Tris-acetic acid-disodium EDTA (TAE) and Tris-boric acid-disodium EDTA (TBE). The main problem of these buffers is that it generates excessive heat, limiting the voltage and speed of electrophoresis. Fast DNA Electrophoresis buffer overcomes this problem. The DNA gels can be run at high voltage (350V for 10cm gels in length) and are ready to be read in 15 minutes. The DNA extracted from the gel can be used for cloning. Usage Dilute in ddH2O to 1x Use diluted buffer to make gel and run the gel Voltage: 10-35V/cm, length of the gel Figure: Gel comprised 1.2% agarose. The gel was run fast (350 V) for 16 min in 1xFEB or 1xTBE. The run used a horizontal gel box,  700 mL total volume of buffers in the reservoirs and gels of 10 cm length. M, DNA marker (1 Kb Plus). PCR, 280-bp PCR fragment.

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Current DNA electrophoresis buffers are mostly restricte to Tris-acetic acid-disodium EDTA (TAE) and Trisboric acid-disodium EDTA (TBE). The main problem of these buffers is that it generates excessive heat, limiting the voltage and speed of electrophoresis. Fast DNA Electrophoresis buffer overcomes this problem. The DNA gels can be run at high voltage (350V for 10cm gels in length) and are ready to be read in 15 minutes. The DNA extracted from the gel can be used for cloning. Usage Dilute in ddH2O to 1x Use diluted buffer to make gel and run the gel Voltage: 10-35V/cm, length of the gel Figure: Gel comprised 1.2% agarose. The gel was run fast (350 V) for 16 min in 1xFEB or 1xTBE. The run used a horizontal gel box,  700 mL total volume of buffers in the reservoirs and gels of 10 cm length. M, DNA marker (1 Kb Plus). PCR, 280-bp PCR fragment.

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TRIS-buffered saline with Tween-20 (20x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS

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TRIS-buffered saline with Tween-20 (20x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS

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TRIS-buffered saline with Tween-20 (20x TBST) is used as a washing buffer for Western blot and other immunoassays, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X TBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl, 0.05% Tween-20 Comment All components except Tween-20 are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. Tween-20 is added and mixed before shipment. MSDS

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TE buffer is a commonly used buffer solution in procedures involving DNA or RNA.  TE buffer functions to solubilize DNA or RNA while protecting it from degradation. This buffer is used at different pH values and has become the standard buffer for the storage of nucleic acids. 1xTE: Tris 10 mM, EDTA 1 mM, pH 7.4

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TE buffer is a commonly used buffer solution in procedures involving DNA or RNA.  TE buffer functions to solubilize DNA or RNA while protecting it from degradation. This buffer is used at different pH values and has become the standard buffer for the storage of nucleic acids. 1xTE: Tris 10 mM, EDTA 1 mM, pH 8.0

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Transfer Buffer (20X) is a buffer used to transfer proteins from gels to membranes for Western blotting.  Composition 1x: Bicine 25 mM, Bis-Tris 25 mM, EDTA 1mM, pH 7.2.

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Transfer Buffer (20X) is a buffer used to transfer proteins from gels to membranes for Western blotting.  Composition 1x: Bicine 25 mM, Bis-Tris 25 mM, EDTA 1mM, pH 7.2.

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Transfer Buffer (20X) is a buffer used to transfer proteins from gels to membranes for Western blotting.  Composition 1x: Bicine 25 mM, Bis-Tris 25 mM, EDTA 1mM, pH 7.2.

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Technical Specs Item Test Tube Rack Mounting Type  Rest on Table Number of Compartments 60 Autoclavable Yes Material Polypropylene Color Blue Lab Rack Capacity Holds 60 test tubes or conical tubes  

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