Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.  Suitable for Bio-Rad® and other systems.  15 wells/gel; 22ul loading capacity Key Features User-friendly gel cassette: Numbered and framed wells for sample loading Labeled warning sign and green tape as reminder Enhanced gel performance:  Enhanced gel electrophoresis speed  Better band separation  Stable for shipping at ambient temperature Easy compatibility:  Available as homogeneous and adjusted gradient gels for a wide range of protein separation. Compatible with most popular protein electrophoresis systems  Storage and stability Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.

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Grade: ULTRA PURE CAS Number: 1185-53-1 Synonym(s): Tris HCl, 2-Amino-2-hydroxymethyl-1 3-propanediol hydrochloride,Tris(hydroxymethyl) aminomethane hydrochloride Molecular Weight: 157.64 Molecular Formula: C4H11NO3.HCl Storage Condition: Room temperature Specification Result Appearance White crystalline Infrafed Pass Purity (By Titration 0.1N NaOH/Dried basis) 99.7% Melt point 150.2-152.8°C Water Content (By Karl Fischer) 0.35% Loss on drying 0.12% pH (1.0% aqueous ) 4.8 Ultraviolet Absorbance (1.0M aqueous) A(260 nm): 0.022A(280 nm): 0.020 Solubility (1.0M aqueous) Clear, colorless solution Heavy metal ≤5ppm Fe ≤5ppm RNase (P/F) None DNase (P/F) None Safety Data Sheet MSDS

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Grade: ULTRA PURE CAS Number: 1185-53-1 Synonym(s): Tris HCl, 2-Amino-2-hydroxymethyl-1 3-propanediol hydrochloride,Tris(hydroxymethyl) aminomethane hydrochloride Molecular Weight: 157.64 Molecular Formula: C4H11NO3.HCl Storage Condition: Room temperature Specification Result Appearance White crystalline Infrared Pass Purity (By Titration 0.1N NaOH/Dried basis) 99.7% Melt point 150.2-152.8°C Water Content (By Karl Fischer) 0.35% Loss on drying 0.12% pH (1.0% aqueous ) 4.8 Ultraviolet Absorbance (1.0M aqueous) A(260 nm): 0.022A(280 nm): 0.020 Solubility (1.0M aqueous) Clear, colorless solution Heavy metal ≤5ppm Fe ≤5ppm RNase (P/F) None DNase (P/F) None Safety Data Sheet MSDS

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Grade: ULTRA PURE CAS Number: 1185-53-1 Synonym(s): Tris HCl, 2-Amino-2-hydroxymethyl-1 3-propanediol hydrochloride,Tris(hydroxymethyl) aminomethane hydrochloride Molecular Weight: 157.64 Molecular Formula: C4H11NO3.HCl Storage Condition: Room temperature Specification Result Appearance White crystalline Infra-red Pass Purity (By Titration 0.1N NaOH/Dried basis) 99.7% Melt point 150.2-152.8°C Water Content (By Karl Fischer) 0.35% Loss on drying 0.12% pH (1.0% aqueous ) 4.8 Ultraviolet Absorbance (1.0M aqueous) A(260 nm): 0.022A(280 nm): 0.020 Solubility (1.0M aqueous) Clear, colorless solution Heavy metal ≤5ppm Fe ≤5ppm RNase (P/F) None DNase (P/F) None Safety Data Sheet MSDS

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Grade: ULTRA PURE CAS Number: 1185-53-1 Synonym(s): Tris HCl, 2-Amino-2-hydroxymethyl-1 3-propanediol hydrochloride,Tris(hydroxymethyl) aminomethane hydrochloride Molecular Weight: 157.64 Molecular Formula: C4H11NO3.HCl Storage Condition: Room temperature Specification Result Appearance White crystalline Infrafed Pass Purity (By Titration 0.1N NaOH/Dried basis) 99.7% Melt point 150.2-152.8°C Water Content (By Karl Fischer) 0.35% Loss on drying 0.12% pH (1.0% aqueous ) 4.8 Ultraviolet Absorbance (1.0M aqueous) A(260 nm): 0.022A(280 nm): 0.020 Solubility (1.0M aqueous) Clear, colorless solution Heavy metal ≤5ppm Fe ≤5ppm RNase (P/F) None DNase (P/F) None Safety Data Sheet MSDS

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Grade: ULTRA PURE CAS Number: 1185-53-1 Synonym(s): Tris HCl, 2-Amino-2-hydroxymethyl-1 3-propanediol hydrochloride,Tris(hydroxymethyl) aminomethane hydrochloride Molecular Weight: 157.64 Molecular Formula: C4H11NO3.HCl Storage Condition: Room temperature Specification Result Appearance White crystalline Infrafed Pass Purity (By Titration 0.1N NaOH/Dried basis) 99.7% Melt point 150.2-152.8°C Water Content (By Karl Fischer) 0.35% Loss on drying 0.12% pH (1.0% aqueous ) 4.8 Ultraviolet Absorbance (1.0M aqueous) A(260 nm): 0.022A(280 nm): 0.020 Solubility (1.0M aqueous) Clear, colorless solution Heavy metal ≤5ppm Fe ≤5ppm RNase (P/F) None DNase (P/F) None Safety Data Sheet MSDS

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Two Step RT-PCR Kit consists of two main components: PowerScript™ Plus cDNA Synthesis MasterMix for reverse transcription 2XTaqPlus PCR MasterMix for subsequent PCR Composition: PowerScript™ Plus Reverse Transcriptase (200U/µl, 30µl) 2xReaction mix contains Ribonuclease Inhibitor, dNTPs, Oligo(dT)s and Random Primers, 350µl Nuclease-free H2O, 1ml  2XTaqPlus PCR MasterMix, 1ml Applications Gene-expression analysis Transcription analysis Gene cloning Multiplex RT-PCR High throughput applications Virus detection and quantification

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Type I grade water, also known as Ultrapure Water, is the purest form of water to be produced. It’s used for the most critical applications and advanced analytical procedures.This includes: Cell and Tissue Cultures Liquid Chromatography, including High Performance Liquid Chromatography (HPLC) Gas Chromatography Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Molecular Biology Type I can also be used in applications that require Type II water. This is quite a common practice that can help to avoid the generation of by-products during applications.

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Type I grade water, also known as Ultrapure Water, is the purest form of water to be produced. It’s used for the most critical applications and advanced analytical procedures. This includes: Cell and Tissue Cultures Liquid Chromatography, including High Performance Liquid Chromatography (HPLC) Gas Chromatography Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Molecular Biology Type I can also be used in applications that require Type II water. This is quite a common practice that can help to avoid the generation of by-products during applications.

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Type I grade water, also known as Ultrapure Water, is the purest form of water to be produced. It’s used for the most critical applications and advanced analytical procedures.This includes: Cell and Tissue Cultures Liquid Chromatography, including High Performance Liquid Chromatography (HPLC) Gas Chromatography Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Molecular Biology Type I can also be used in applications that require Type II water. This is quite a common practice that can help to avoid the generation of by-products during applications.

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The Ultrasense Pico Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to mid femtogram detection of antigen is enabled by Ultrasense Pico Ultra Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duratio Features:  No optimization required. Switching to the Ultrasense Pico Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ West Dura and Millipore™ Immobilon™ substrates, does not require optimization or protocol changes. High degree of sensitivity and enhanced chemiluminescence duration. UltraSense Pico Western Substrate enables an accurate low picogram to mid femtogram detection of protein on the same immunoblot after a single exposure. Optimized for use with PVDF and nitrocellulose membranes Compatible with Western Blotting Markers Optimized for film- and CCD-based imaging. Chemiluminescent development Keep the membrane moist in the wash buffer while preparing the substrate mixture. Make sure the membrane does not dry out in the next steps. The chemiluminescent substrate solution is sufficiently stirred to prepare the 0.1ml of solution / cm2 of the membrane. For a small membrane (7 x 8.5 cm), 4 ml of solution is sufficient. For a medium-sized membrane (8.5 x 135 cm), 10 ml of solution is sufficient. Place the membrane on a clean, flat surface or in a clean container. Remove the membrane from the chemiluminescent substrate solution and drain the excess solution. Place the membrane in a plastic sheet protector or plastic wrap to prevent the film from drying out. Imaging the membrane with a digital imager or by exposure to the X-ray film. 

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The Ultrasense Pico Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to mid femtogram detection of antigen is enabled by Ultrasense Pico Ultra Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duratio Features:  No optimization required. Switching to the Ultrasense Pico Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ West Dura and Millipore™ Immobilon™ substrates, does not require optimization or protocol changes. High degree of sensitivity and enhanced chemiluminescence duration. UltraSense Pico Western Substrate enables an accurate low picogram to mid femtogram detection of protein on the same immunoblot after a single exposure. Optimized for use with PVDF and nitrocellulose membranes Compatible with Western Blotting Markers Optimized for film- and CCD-based imaging. Chemiluminescent development Keep the membrane moist in the wash buffer while preparing the substrate mixture. Make sure the membrane does not dry out in the next steps. The chemiluminescent substrate solution is sufficiently stirred to prepare the 0.1ml of solution / cm2 of the membrane. For a small membrane (7 x 8.5 cm), 4 ml of solution is sufficient. For a medium-sized membrane (8.5 x 135 cm), 10 ml of solution is sufficient. Place the membrane on a clean, flat surface or in a clean container. Remove the membrane from the chemiluminescent substrate solution and drain the excess solution. Place the membrane in a plastic sheet protector or plastic wrap to prevent the film from drying out. Imaging the membrane with a digital imager or by exposure to the X-ray film. 

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