Features Universal fit: Eppendorf, Gilson Sterile DNase/RNase Free 50 racks/cs, 96 tips/rack, 4,800 tips

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PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 80 mM Na2HPO4, and 20 mM KH2PO4 Suitable For Western blot, ELISA, immunoprecipitation Comment All components are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter MSDS

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PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 80 mM Na2HPO4, and 20 mM KH2PO4 Suitable For Western blot, ELISA, immunoprecipitation Comment All components are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter MSDS

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PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. Each 10X PBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 80 mM Na2HPO4, and 20 mM KH2PO4 Suitable For Western blot, ELISA, immunoprecipitation Comment All components are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter MSDS

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PBST (phosphate buffered saline with Tween-20) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer and 0.05% Tween-20. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X PBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 100mM phosphate buffer and 0.5% Tween-20 Suitable For Western blot, ELISA, immunoprecipitation Comment All components except Tween-20 are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter. Tween-20 is added and mixed before shipment. MSDS

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PBST (phosphate buffered saline with Tween-20) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer and 0.05% Tween-20. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X PBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 100mM phosphate buffer and 0.5% Tween-20 Suitable For Western blot, ELISA, immunoprecipitation Comment All components except Tween-20 are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter. Tween-20 is added and mixed before shipment. MSDS

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PBST (phosphate buffered saline with Tween-20) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions which, when diluted to a 1X working concentration, contains 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer and 0.05% Tween-20. Tween 20 reduces nonspecific binding and protein: protein interactions. Each 10X PBST solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2, after 1X dilution with DI water Composition 1.37 M NaCl, 27 mM KCl, 100mM phosphate buffer and 0.5% Tween-20 Suitable For Western blot, ELISA, immunoprecipitation Comment All components except Tween-20 are dissolved in ultrapure (type I) water, adjusted pH and filtered with 0.45µm filter. Tween-20 is added and mixed before shipment. MSDS

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10X TBE Buffer (Tris-borate-EDTA) is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer is recommended when looking at enzymatic applications for the DNA sample. Applications Electrophoresis of nucleic acids in agarose and polyacrylamide gels Used both as a running buffer and as a gel preparation buffer Filtered through a 0.45 μm membrane Recommended for electrophoresis of RNA and DNA fragments smaller than 1500 bp Composition 10x: Tris 890 mM, Boric acid 890 mM, EDTA 20 mM, pH 8.3 MSDS

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10X TBE Buffer (Tris-borate-EDTA) is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer is recommended when looking at enzymatic applications for the DNA sample. Applications Electrophoresis of nucleic acids in agarose and polyacrylamide gels Used both as a running buffer and as a gel preparation buffer Filtered through a 0.45 μm membrane Recommended for electrophoresis of RNA and DNA fragments smaller than 1500 bp Composition 10x: Tris 890 mM, Boric acid 890 mM, EDTA 20 mM, pH 8.3 MSDS

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10X TBE Buffer (Tris-borate-EDTA) is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer is recommended when looking at enzymatic applications for the DNA sample. Applications Electrophoresis of nucleic acids in agarose and polyacrylamide gels Used both as a running buffer and as a gel preparation buffer Filtered through a 0.45 μm membrane Recommended for electrophoresis of RNA and DNA fragments smaller than 1500 bp Composition 10x: Tris 890 mM, Boric acid 890 mM, EDTA 20 mM, pH 8.3 MSDS

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TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.4 ± 0.2 Composition (1x) 50mM Tris, 150mM NaCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter. MSDS

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TRIS-buffered saline (10x TBS) is used as a washing buffer for Western blot and other alkaline phosphatase and peroxidase conjugates assay, such as ELISA and dot blot assay. It acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions. Each 10X TBS solution is ready to use upon dilution to the desired concentration.  Product Specification Appearance Clear, Colorless Form Liquid pH 7.6 ± 0.2 Composition (1x) 25mM Tris, 137mM NaCl, 2.7mM KCl Comment All components are dissolved in ultrapure (Type I) water, followed by pH adjustment and filtered through 0.45 micron filter.  MSDS

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