Tank Vent Filter with CO2 scavenger RATANKVN7 is designed to prevent airborne particles, CO2, organic and bacteria from entering the PE water tank. It contains a scavenger to remove CO2.
Purification Technologies:
Activated Carbon
Membrane Filtration
Chemistry Adsorption
Technical Specifications:
Height
393.8mm
Diameter
φ90mm
Suitable for:
30 / 60 / 100 L Genie tank
5 U/µl (1µl contains 5 units Taq DNA Polymerase)
Amplify DNA target up to 6 kb
Elongation velocity is 1kb/min
Lacks of 3' to 5' exoneclease activity
Makes 3'-dA overhangs PCR product, good for TA cloning
10x Reaction Buffer is included
A novel recombinant reverse transcriptase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. PowerScriptTM Plus Reverse Transcriptase is engineered to perform under high temperatures (50°C - 60°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, ThermoScriptTM Plus can synthesize full-length cDNA libraries from RNA templates up to 12 kb in length.
Features
Lack RNase H activity: higher yield of cDNA.
Thermal stable: the optimum reaction temperature is 50°C, the highest is 60°C. Can overcome the template RNA secondary structure, and finish the reverse transcriptase experiment smoothly.
Wide temperature range: can reverse transcript from 37-60°C, with more than 80% of the highest activity at 42°C-60°C. customer can choose the reaction temperature freely.
Strong amplification activity: Gene mutation enhanced the binding capacity of the enzyme and RNA. Increased the amplification speed, can obtain the quality cDNA, suitable for cDNA library construction.
Applications
Synthesizing cDNA from a ssRNA
Constructing cDNA library
Source
Recombination of E. coli containing Moloney murine leukemia virus reverse transcriptase gene from clone of Moloney murine.
Unit definition
Using Poly (A) as the template and oligo (dT) as the primer, the enzyme required to catalyze the incorporation of 1 nmol of dTTP within 10 minutes at 37 oC is defined as one active unit (U).
Concentration: 200 U/ul
Size: 100 Reactions (125 ul)
Form: Enzyme supplied with 5X RT buffer
Storage: Store all components at -20°C
THM-INCU Microplate incubator is a microprocessor and PID controlled incubator with high performance. It accommodates up to two microplates. The incubator can be used for any enzyme or cell-based assays requiring uniform and strictly controlled temperature up to 70℃. It's compatible with coated plates (96-, 384-well plates) or tissue culture plates.
Features
Heated lid prevents condensation
Accepts a variety of microplates
Easy to operate with one touch knob
Specifications
Temperature Control Range
RT+5℃~70℃
Timing Range
1min~99h59min
Temperature Control Accuracy
≤±0.5℃
Display Accuracy
0.1℃
Heating Speed
≤25min
Capacity
2 microplate
Power
150-200w
Dimensions( Lx W x H)mm
280 x 270 x 140mm
Weight
7kg
THMS Heating Microplates Shaker applies the technologies of brushless DC motor and PID intelligent temperature controI. It's mainly used for assays using ELISA plates (96-, 384-wells), and tissue culture plates.
Features:
3mm Mixing orbit
Heated lid prevents condensation
Accepts a variety of microplates
Easy to operate with one touch knob
Specifications
Incubation
8℃~70℃
Display Accuracy
0.1℃ steps
Temperature stability
±0.3℃/ Temperature stability ±0.3℃
Warming Up Speed
<25min(form 20℃ to 70℃)
Shaking
1.5mm orbit radius
Shaking range form 200 to 1600rpm(increment:1rpm)
Timed shaking
range:0 to 99hours 59min in 1min steps
Continuous shaking - no time limit
Capacity
2 microplates
Power Requirements
110v, 60/50Hz,max.150VA
Dimensions
280 x 270 x 140mm
The EasyPrepTM DNA/RNA Kit provides a simple and rapid method for simultaneously isolating genomic DNA and total RNA from a biological samples, which includes cultured cells, tissues, whole blood, plasma, serum, and body fluids. This co-purification system saves time on sample handling, avoids the sample variation and saves precious samples, which is essential when the amount of available samples are limited
Instead of using proteinase K to digest the biological materials, the EasyPrepTM DNA/RNA Kit applies the lysis buffer directly to the cells or tissues. After homogenization, the lysates are transferred to DNA columns. Thus the time-consuming steps of material preparation are eliminated. The DNA-binding-specific matrix in the DNA column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are passed through the DNA columns and are collected. Genomic DNA are then purified from the DNA columns, while total RNA are purified from the collected eluate through RNA binding columns. Purified DNA can be directly used for downstream applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion, while purified total RNA can be used for RT-PCR, Northern blotting or microarray analysis.
Kit's Contents
SKU
DR01-01
DR01-02
Storage
Preps
50
250
-
DNA columns
50
250
RT
RNA columns
50
250
RT
GD Lysis Buffer
60 ml
260 ml
RT
DNA Wash Buffer
12 ml
50 ml
RT
DNA Elution Buffer
10 ml
25 ml
RT
RNA Wash Buffer
12 ml
50 ml
RT
DEPC H2O
10 ml
25 ml
RT
Collection tubes
100
500
RT
Manual
1
1
-
The EasyPrepTM DNA/RNA Kit provides a simple and rapid method for simultaneously isolating genomic DNA and total RNA from a biological samples, which includes cultured cells, tissues, whole blood, plasma, serum, and body fluids. This co-purification system saves time on sample handling, avoids the sample variation and saves precious samples, which is essential when the amount of available samples are limited
Instead of using proteinase K to digest the biological materials, the EasyPrepTM DNA/RNA Kit applies the lysis buffer directly to the cells or tissues. After homogenization, the lysates are transferred to DNA columns. Thus the time-consuming steps of material preparation are eliminated. The DNA-binding-specific matrix in the DNA column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are passed through the DNA columns and are collected. Genomic DNA are then purified from the DNA columns, while total RNA are purified from the collected eluate through RNA binding columns. Purified DNA can be directly used for downstream applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion, while purified total RNA can be used for RT-PCR, Northern blotting or microarray analysis.
Kit's Contents
SKU
DR01-01
DR01-02
Storage
Preps
50
250
-
DNA columns
50
250
RT
RNA columns
50
250
RT
GD Lysis Buffer
60 ml
260 ml
RT
DNA Wash Buffer
12 ml
50 ml
RT
DNA Elution Buffer
10 ml
25 ml
RT
RNA Wash Buffer
12 ml
50 ml
RT
DEPC H2O
10 ml
25 ml
RT
Collection tubes
100
500
RT
Manual
1
1
-
The EasyPrepTM DNA/RNA Kit provides a simple and rapid method for simultaneously isolating genomic DNA and total RNA from a biological samples, which includes cultured cells, tissues, whole blood, plasma, serum, and body fluids. This co-purification system saves time on sample handling, avoids the sample variation and saves precious samples, which is essential when the amount of available samples are limited
Instead of using proteinase K to digest the biological materials, the EasyPrepTM DNA/RNA Kit applies the lysis buffer directly to the cells or tissues. After homogenization, the lysates are transferred to DNA columns. Thus the time-consuming steps of material preparation are eliminated. The DNA-binding-specific matrix in the DNA column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are passed through the DNA columns and are collected. Genomic DNA are then purified from the DNA columns, while total RNA are purified from the collected eluate through RNA binding columns. Purified DNA can be directly used for downstream applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion, while purified total RNA can be used for RT-PCR, Northern blotting or microarray analysis.
Kit's Contents
SKU
DRP01-01
DRP01-02
Storage
Preps
50
250
-
DNA columns
50
250
RT
RNA columns
50
250
RT
GD Lysis Buffer
60 ml
260 ml
RT
DNA Wash Buffer
12 ml
50 ml
RT
DNA Elution Buffer
10 ml
25 ml
RT
RNA Wash Buffer
12 ml
50 ml
RT
DEPC H2O
10 ml
25 ml
RT
Collection tubes
100
500
RT
Protein Isolation Buffer
40 ml
200 ml
RT
Manual
1
1
-
The EasyPrepTM DNA/RNA Kit provides a simple and rapid method for simultaneously isolating genomic DNA and total RNA from a biological samples, which includes cultured cells, tissues, whole blood, plasma, serum, and body fluids. This co-purification system saves time on sample handling, avoids the sample variation and saves precious samples, which is essential when the amount of available samples are limited
Instead of using proteinase K to digest the biological materials, the EasyPrepTM DNA/RNA Kit applies the lysis buffer directly to the cells or tissues. After homogenization, the lysates are transferred to DNA columns. Thus the time-consuming steps of material preparation are eliminated. The DNA-binding-specific matrix in the DNA column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are passed through the DNA columns and are collected. Genomic DNA are then purified from the DNA columns, while total RNA are purified from the collected eluate through RNA binding columns. Purified DNA can be directly used for downstream applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion, while purified total RNA can be used for RT-PCR, Northern blotting or microarray analysis.
Kit's Contents
SKU
DRP01-01
DRP01-02
Storage
Preps
50
250
-
DNA columns
50
250
RT
RNA columns
50
250
RT
GD Lysis Buffer
60 ml
260 ml
RT
DNA Wash Buffer
12 ml
50 ml
RT
DNA Elution Buffer
10 ml
25 ml
RT
RNA Wash Buffer
12 ml
50 ml
RT
DEPC H2O
10 ml
25 ml
RT
Collection tubes
100
500
RT
Protein Isolation Buffer
40 ml
200 ml
RT
Manual
1
1
-
The EasyPrepTM Genomic DNA Kit provides a simple and rapid method for the isolation of genomic DNA from a wide variety of biological materials, including cultured cells, tissues, whole blood, plasma, serum, and body fluids.
Instead of using proteinase K to digest the biological materials, the kit applies the lysis buffer directly to the cells or tissues. After homogenization, the lysates are transferred to the binding columns. Thus the time-consuming steps of material preparation are eliminated. The DNA-binding-specific matrix in the column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are easily removed during washing. Purified DNA can be directly used for most applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion.
Kit's Contents
SKU
GD01-01
GD01-02
Storage
Preps
50
250
-
DNA columns
50
250
RT
GD Lysis Buffer
60 ml
260 ml
RT
DNA Wash Buffer
12 ml
50 ml
RT
DNA Elution Buffer
10 ml
25 ml
RT
Collection tubes
50
250
RT
Manual
1
1
The EasyPrepTM Genomic DNA Kit provides a simple and rapid method for the isolation of genomic DNA from a wide variety of biological materials, including cultured cells, tissues, whole blood, plasma, serum, and body fluids.
Instead of using proteinase K to digest the biological materials, the kit applies the lysis buffer directly to the cells or tissues. After homogenization, the lysates are transferred to the binding columns. Thus the time-consuming steps of material preparation are eliminated. The DNA-binding-specific matrix in the column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are easily removed during washing. Purified DNA can be directly used for most applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion.
Kit's Contents
SKU
GD01-01
GD01-02
Storage
Preps
50
250
-
DNA columns
50
250
RT
GD Lysis Buffer
60 ml
260 ml
RT
DNA Wash Buffer
12 ml
50 ml
RT
DNA Elution Buffer
10 ml
25 ml
RT
Collection tubes
50
250
RT
Manual
1
1
Introduction
The tissue homogenizer utilizes a vertical shock system with high-speed reciprocating motion to make the frozen samples in the grinding tube collide with the grinding beads, The resulting grinding shear force and impact force completely crush the tissues, making it a specialized device for rapid one-time processing of multiple samples in research institutions, universities and agricultural, biomedical, food testing fields.
Features:
Linear guide rail, stable and durable, capable of continuous grinding for 24 hours
Capable of processing up to 24 samples at a time
Adapter module can be pre-cooled at low temperature to reduce sample degradation
Multiple adapters are optional
Compact and sleek appearance
Parameter settings and reference for grinding beads
Tissues
Grinding Beads
Grinding Time
Pause Time
Cycle Number
Frequency
Liver and Spleen
4mm*13mm*1
30s
15s
2
60Hz
Muscle
4mm*23mm*2
45s
15s
2
60Hz
Skin
4mm*23mm*3
60s
20s
4
70Hz
Blood Vessel
4mm*23mm*2
60s
15s
3
70Hz
Aorta
4mm*23mm*3
60s
15s
4
70Hz
Soft Bone
4mm*23mm*3
60s
20s
5
70Hz
Plant Leaf
4mm*13mm*2
45s
15s
3
60Hz
Plant Stem
4mm*13mm*2
60s
15s
3
60Hz
Hair
4mm*23mm*3
90s
20s
4
70Hz
Hard Bone
4mm*23mm*3
90s
30s
5
70Hz
*It is recommended to use steel grinding beads for protein extraction and zirconia grinding beads for RNA extraction.
Notes
When using 4mm grinding beads or large grinding beads, it is necessary to use a 2mL U-bottom tube. 1.5mL conical tube may cause obstruction, affecting the grinding effect (it is recommended to use our 2ml centrifuge tubes, which have thicker walls and higher material strength, suitable for grinding, SKU: TUBE0020-H).
The tissue size is preferably below 1-3mm, and the weight should be within 100mg.
If you want to reuse the grinding beads, it is recommended to soak them in absolute ethanol for 15 minutes before use, then filter out the grinding beads and dry them for future use.
To prevent sample degradation while ensuring the grinding effect, the following principles should be followed:
Minimize the number of grinding beads used
Minimize the grinding time
When using the instrument, ensure that the load on both sides of the adapter is balanced, and do not use it unilaterally.
To protect the internal circuitry and mechanics of the instrument, do not rinse it with running water. Use a damp cloth to clean it.
If any abnormality occurs during use, immediately disconnect the power and contact a professional for handling.
The instrument must be placed on a horizontal and flat laboratory bench to prevent vibration during operation.
After ensuring that the adapter and fastening screws are correctly installed, close the top cover and start the instrument.