The EasyPrepTM Genomic DNA Kit provides a simple and rapid method for the isolation of genomic DNA from a wide variety of biological materials, including cultured cells, tissues, whole blood, plasma, serum, and body fluids.
Two protocols are used in this manual, the traditional method is to lyse the red blood cells and remove the debris. It prevents the debris to clog the DNA columns. Thus can be used for larger volume of whole blood samples. The second method is to apply the lysis buffer directly to the whole blood, serum or plasma, which saves time. This method is especially good for small volume blood samples.
After homogenization, the lysates are transferred to the binding columns. The DNA-binding-specific matrix in the column allows the high efficient binding of DNA, while RNA, proteins and other cellular components are easily removed during washing. Purified DNA can be directly used for most applications such as PCR, Southern blotting, sequencing and restriction enzyme digestion.
Kit's Contents
| SKU |
GD02-01 |
GD02-02 |
Storage |
DNA column set
|
50 |
250 |
RT |
| GD Lysis Buffer |
60 ml |
260 ml |
RT |
| DNA Wash Buffer (5x concentrate) |
12 ml |
54 ml |
RT |
| DNA Elution Buffer |
10 ml |
25 ml |
RT |
| Column Buffer |
10 ml |
30 ml |
RT |
| Red Blood Cell Lysis Solution* |
40 ml |
200 ml |
RT |
| Manual |
1 |
1 |
|
*Lysis Buffer contains Chaotropic salts. Wear gloves when handling this solution
