{"product_id":"5m-betaine-solution-molecular-biology-grade","title":"5M Betaine Solution (Molecular biology grade)","description":"\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eGeneral description\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eBetaine also called trimethylglycine or \u003ci\u003eN,N,N\u003c\/i\u003e triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the dsDNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘long and accurate’ polymerase chain reaction (LA-PCR). The addition of 1-2 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eA\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003epplication\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eBetaine solution has been used as a component of loop-mediated isothermal amplification (LAMP) reactions polymerase chain reaction for genomic DNA amplification quantitative polymerase chain reaction (qPCR) for telomere length analysis in the PCR amplification of CGG repeats in the genomic DNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul style=\"margin-top: 0in;\" type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo1; tab-stops: list .5in;\"\u003e\n\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eFinal Reaction Concentration:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e Most protocols use \u003cb\u003e1.0 M to 1.5 M\u003c\/b\u003e as a starting point, which can be optimized up to \u003cb\u003e2.0 M\u003c\/b\u003e for particularly difficult, GC-rich targets or templates that form tight secondary structures.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo1; tab-stops: list .5in;\"\u003e\n\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eVolume Calculation (using a 5M stock solution):\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e If you are setting up a standard 20ul or 50ul PCR reaction, you will add enough of your \u003cb\u003e5M betaine\u003c\/b\u003e solution to hit that final 1 M–2 M concentration. For example:\u003c\/span\u003e\n\u003cul style=\"margin-top: 0in;\" type=\"circle\"\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level2 lfo1; tab-stops: list 1.0in;\"\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eTo get \u003cb\u003e1.0 M final concentration\u003c\/b\u003e in a 20ul reaction, you need to add 4ul of a 5M betaine stock.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level2 lfo1; tab-stops: list 1.0in;\"\u003e\n\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eTo get \u003cb\u003e1.5 M final concentration\u003c\/b\u003e in a 20ul reaction, you need to add 6ul of a 5M betaine stock.\u003c\/span\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eStorage:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cul style=\"margin-top: 0in;\" type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo1; tab-stops: list .5in;\"\u003e\n\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eShort-Term Storage:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e Store at 4\u003csup\u003eo\u003c\/sup\u003eC (refrigerated) for daily or weekly use.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo1; tab-stops: list .5in;\"\u003e\n\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eLong-Term Storage:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e Aliquot the solution into smaller volumes and store at -20\u003csup\u003eo\u003c\/sup\u003eC to prevent contamination and repeated freeze-thaw cycles.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003eShipping:\u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-family: 'Arial',sans-serif;\"\u003e 5M betaine is a stable aqueous solution and does not require cold-chain (ice or dry ice) shipping. It can be shipped at ambient room temperature.\u003c\/span\u003e\u003c\/p\u003e","brand":"Bioland Scientific LLC","offers":[{"title":"1.4ml","offer_id":48823278796989,"sku":null,"price":12.0,"currency_code":"USD","in_stock":true},{"title":"5x 1.4ml","offer_id":48823278829757,"sku":null,"price":50.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/1885\/6125\/files\/Betaine.jpg?v=1789861275","url":"https:\/\/bioland-sci.com\/products\/5m-betaine-solution-molecular-biology-grade","provider":"Bioland Scientific LLC","version":"1.0","type":"link"}