Tri-color Prestained Protein Marker II (10-245kD), 0.5 ml

Price: $98.00

  • The ladder is supplied in gel loading buffer and is ready to use.
  • 3  μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
  • 1.5~2.5 μl per well for general Western transferring.
  • Apply more for thicker (> 1.5 mm) or larger gel.

Description:  The Tri-colorPrestained Protein Ladder II is a three-color protein standard with 12pre-stained proteins covering a wide range molecular weightsfor 10 to 245kDa.Proteins are covalently coupled with a blue chromophore except for tworeference bands (one green and one red band at 25 kDa and 75 kDarespectively) when separated on SDS-PAGE (Tris-glycine buffer). The Tri-color Prestained Protein LadderIIis designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.

Contents: Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 3.6M Urea, and 15 % (v/v) Glycerol).

Quality Control: Under suggested conditions, Tri-color Prestained Protein Ladder I resolves12major bands in 15% SDS-PAGE (Tris-glycine buffer)andafterWestern blotting to nitrocellulose membrane.

Storage: Stable at 4oC for 3 months. For long term storage, store at -20oC. 

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  • Shipping Weight: 15lbs

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