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Soil genomic DNA miniprep kit (50 Preps)

Price: $90.00

The EasyPrepTM Soil gDNA Kit is designed for a rapid and reliable purification of high-quality genomic DNA from various soil samples. Up to 1 gram  of  soil samples can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of ezBind™ matrix with a propriety buffer system to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands- on time to allow multiple samples to be processed in parallel.

In this procedure, soil sample is homogenized and then treated in a specially formulated buffer that contains detergent. Humic acid, proteins, polysaccharides, and other contaminants are subsequently precipitated after a heat-frozen step. DNA is further purified with a DNA spin-column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in water or low ionic strength Elution Buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Storage and Stability
All components of the EasyPrepTM Soil gDNA Kit should be stored at 22oC-25oC. Under these conditions, DNA has been successfully purified and used for PCR after 12 months of storage. During shipment, or storage in cool ambient conditions, precipitates may form in some buffers. It is possible to dissolve such deposits by incubation the solution at 65oC.

Kit Content:

Catalog #
GD2412-01
GD2412-02
Preps
50
250
DNA columns
50
250
2 mL collection tubes
100
500
Glass beads
12g
55g
DH reagent
12 mL
60 mL
Buffer LX
40 mL
200 mL
Buffer P2
15 mL
60 mL
Buffer BL
30 mL
150 mL
Elution Buffer
20 mL
100 mL
DNA Wash Buffer
20 mL
3 x 24 mL
RNase A
160 μL
800 μL
Instruction Booklet
1
1

Protocol:

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Add to Cart:

  • Model:
    [GD2412-01]
  • Shipping Weight: 5lbs

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