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Endofree plasmid maxiprep kit (10 Preps)

Price: $145.00

EasyPrep EndoFree Plasmid Maxiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 1,000 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming.

Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections.

Kit Content

Catalog #

PD05-21
PD05-31
PD05-22
PD05-32
Maxiprep DNA Columns
10
25
ezFilter
10 (PD05-31)
25 (PD05-32)
Buffer A1
120 ml
270 ml
Buffer B1
120 ml
270 ml
Buffer N3
60 ml
150 ml
Buffer RET
120 ml
270 ml
DNA Wash Buffer (5x)*
54 ml
2x 54 ml
EndoFree Elution Buffer (H2O)
20 ml
30 ml
RNase A
420 µl
920 µl
Manual
1
1

Protocol

Image result for pdf image


Add to Cart:

  • Model:
    [PD05-21]
  • Shipping Weight: 5lbs

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