50x TAE Buffer (4 L)

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1x: Tris 40 mM, Acetate 40 mM, EDTA 1 mM, pH 8.0
50X TAE Buffer (Tris-acetate-EDTA) is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA.
  • Electrophoresis of nucleic acids in agarose and polyacrylamide gels
  • Used both as a running buffer and as a gel preparation buffer
  • Filtered through a 0.22 µm membrane
  • Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA
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