Endofree plasmid midiprep kit (25 Preps)

Price: $185.00  $160.00
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EasyPrep EndoFree Plasmid Midiprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs from bacterial culture, when amount of the plasmid DNA needed is about 200 µg. The kit is especially convenient to use when multiple plasmids need to be purified at the same time, as the use of traditional commercial kits is time-consuming.

Key to the kit is our proprietary DNA binding systems that allow the high efficient DNA binding  while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with Elution Buffer. Our proprietary endotoxin removal buffer, Buffer RET, is designed to remove endotoxin by a single washing step without tedious phase partitioning steps. The purified plasmid DNA, with endotoxin level less than 0.1 EU per µg of DNA, is ready for transfection of endotoxin sensitive cell lines and microinjections.

Kit Content

Catalog #

Midiprep DNA Columns
10 (PD03-31)
25 (PD03-32)
Buffer A1
30 ml
75 ml
Buffer B1
30 ml
75 ml
Buffer N3
15 ml
30 ml
Buffer RET
30 ml
75 ml
DNA Wash Buffer (5x)*
25 ml
50 ml
EndoFree Elution Buffer
10 ml
20 ml
RNase A
110 µl
250 µl


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