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Tri-color Prestained Protein Marker I (10-180kD), 5x0.5 ml

Price: $396.00

  • 3 μl or 5 μl per loading for clear visualization during electrophoresis
    on 15-well or 10-well mini-gel, respectively.
  • 1.5~2.5 μl per well for general Western transferring.
  • Apply more for thicker (> 1.5 mm) or larger gel.
  • The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, or add reducing agent before loading.

Description: The Tri-colorPrestained Protein Ladder I is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weightsfor 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for tworeference bands (one green and one red band at 25 kDa and 75 kDarespectively) when separated on SDS-PAGE (Tris-glycine buffer). The Tri-color Prestained Protein LadderIis designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.

Contents: Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM 2-mercaptoethanol, 3.6M Urea, and 15 % (v/v) Glycerol).

Quality Control: Under suggested conditions, Tri-color Prestained Protein Ladder I resolves10 major bands in polyacrylamide gel with appropriate buffers andafterWestern blotting to nitrocellulose membrane.

Storage:

  • Stable at 25oC for 2 weeks
  • Stable at 4oC for 3 months
  • Stable at -20oC for 24 months

Add to Cart:

  • Model:
    [PM01-02]
  • Shipping Weight: 4lbs

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