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Tri-color Extra Range Protein Marker (3.5-245kD), 5x0.5 ml

Price: $540.00

  • The ladder is supplied in gel loading buffer and is ready to use.
  • 3  μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
  • 1.5~2.5 μl per well for general Western transferring.
  • Apply more for thicker (> 1.5 mm) or larger gel.

The Tri-color Protein Marker III is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 3.5 to 235 kDa in MOPS buffer and MES buffer or 5 to 245 kDa in Tris-Glycine buffer, . Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The Tri-color Prestained Protein Marker III is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).

Quality Control
Under suggested conditions, Tri-color Protein Marker III resolves 13 major bands in SDS-PAGE (Bis-Tris gel, MES buffer) and after Western blotting to nitrocellulose membrane.

Storage
4°C for 3 months
-20°C for 24 months
 


Add to Cart:

  • Model:
    [PM03-02]
  • Shipping Weight: 15lbs

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