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All Blue Prestained Protein Marker II (10-245kD), 5x0.5 ml

Price: $337.50

  • 3 μl or 5 μl per loading for clear visualization during electrophoresis
    on 15-well or 10-well mini-gel, respectively.
  • 1.5~2.5 μl per well for general Western transferring.
  • Apply more for thicker (> 1.5 mm) or larger gel.
  • The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, or add reducing agent before loading.

Description
All Blue Broad Range Protein Marker II is a blue protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 240 kDa in Tris-Glycine buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer).  All Blue Broad Range Protein Marker II is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Contents
Approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).

Quality Control
Under suggested conditions, All Blue Broad Range Protein Marker II resolves 12 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane.

Storage

  • Stable at 25oC for 2 weeks
  • Stable at 4oC for 3 months
  • Stable at -20oC for 24 months

Add to Cart:

  • Model:
    [PM05-02]
  • Shipping Weight: 3lbs

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