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5-Layer Cell Culture Flask (Vent Cap, TC, 1/pk, case of 8)

Price: $145.00

Features:

  • High clarity medical grade polystyrene material
  • High performance with 5 growth surfaces
  • Growth area: 870cm2
  • TC treatment for better cell attachment
  • Sterilized by E-Beam, non-pyrogenic
  • Vent cap

User Instruction

ADDING MEDIA AND PREPARING CELL SUSPENSION

  1. Mix cell suspension with medium: Prepare cell suspension of required concentration in a container and mix. Recommended volume is about 30- 50 mL per layer.
  2. Add the mixed liquid into the Multi-layer Flask slowly with serological pipettes. To avoid foams and bubbles, allow liquid stream to flow along the slope of the Multi-layer Flask. (Save a little liquid in pipette for each dosing)

Tips: A 10 mL pipette allows media to be dispensed at the bottom of the vessel. A 25 mL pipette allows media to be dispensed just past the NEST Logo.

  1. Hold the Multi-layer Flask upright with the Logo facing you and tilt clockwise to a 45° angle on a flat work surface to partition the liquid into each layer.
  2. While holding the Multi-layer Flask at a 45° angle, gently lay it flat onto the work surface with logo facing up.
  3. After placing the Multi-layer Flask flat on a work surface, gently rock back and forth and side-to-side to distribute cells evenly onto culture surfaces.
  4. Repeat Step 3 to put the flask quickly and slightly into the incubator . Then, lay it flat as shown in Step 4 .

MEDIA REMOVAL

  1. Aspirating method: To aspirate or remove media, tilt Multi-layer Flask, with the NEST Logo facing you, counter-clock wise to a 45° angle while inverting the Multi-Flask toward you. Then, tilt Multi-layer Flask to the right, continuing to aspirate all residual media.
  2. Pouring method: With Logo facing you, pour spent media from Multi-layer Flask

CELL HARVESTING

  1. Wash with buffer for one time and add dissociating reagent (≥5 mL per layer). Then, follow Steps 3-4 to distribute to dissociating reagent to each layer.
  2. Neutralize with inactivating solution and mix following Steps 3-4. Gently swirl to dislodge cells completely.
  3. Follow Step 7 “Aspirating Method” protocol and collect cell suspension using a NEST 10mL serological pipet.
  4. Follow Step 8 “Pouring Method”. Pour de¬tached cell suspension into a NEST conical tube. 13. Rinse with additional wash buffer as needed.

 


Add to Cart:

  • Model:
    [731002]
  • Shipping Weight: 13lbs

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