DNase I (RNase-Free), 100 mg

Price: $55.00

Specific Activity: ≥ 2000 Kunitz units/mg
Form: Lyophilized Powder
Storage: 4oC
DNase Ⅰ (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.
  • Recombinant enzyme
  • Purified from non-animal host with a lower level of intrinsic RNases.
  • Degradation of DNA template in transcription reactions
  • Removal of contaminating genomic DNA from RNA samples
  • DNase I footprinting
  • Nick Translation
Quality Control
The absence of ribonucleases is confirmed by appropriate quality test. Functionally tested for digestion of template DNA after in vitro transcription
Source: A E. Coli strain that carries an MBP fusion clone of Bovine Pancreatic DNase I.
Molecular Weight: 29 kDa monomer
Definition of Activity Unit
One unit of the enzyme completely degrades 1 μg of plasmid DNA in 10 min at 37oC. Enzyme activity is assayed in the following mixture: 40 mM Tris-HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2, 1 μg of pUC19 DNA.
Inhibition and Inactivation
  • Inhibitors: metal chelators, transition metals (e.g., Zn) in millimolar concentrations, SDS (even at concentrations less than 0.1%), reducing agents (DTT and beta-mercaptoethanol), ionic strength above 50-100 mM.
  • Inactivated by heating at 65oC for 10 min in the presence of EGTA or EDTA (use at least 1 mol of EGTA/EDTA per 1 mol of Mn2+/Mg2+ ).
Note: DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not Vortex.

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