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Pfu DNA polymerase (1,000 U)

Price: $175.00

PowerPCR Pfu DNA polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus.

  • 2.5 U/µl
  •  Amplify DNA target up to 2 kb
  • The elongation velocity is 0.2~0.4kb/min (70~75°C).
  • 6-10 fold higher fidelity than Taq DNA polymerase
  • Highly thermostable – remains 95% active after 2 hours incubation at 95°C
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
  • Possesses 3' to 5' exonuclease proofreading activity
  • Generates in blunt-ended PCR products

Applications

  • High fidelity PCR
  • Generation of PCR products for cloning and expression
  • RT-PCR for cDNA cloning and expression
  • Blunt-end PCR cloning

Add to Cart:

  • Model:
    [E03-03]
  • Shipping Weight: 20lbs

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