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BioT, FuGene-6 and Lipofectamine-2000

 
 Cost comparison (based on standard usage)
Culture dish
Volume Of
Plating Medium
FuGENE-6
$346/1.0ml
Lipofectamine-2000
$457/1.5ml
BioT
$320/1.0ml
 
10 cm
10 ml
30 µl
$10.38
40 µl
$12.19
15 µl
$4.80
60 mm
3 ml
9 µl
$3.11
12 µl
$3.66
4.5 µl
$1.44
35 mm
2 ml
6 µl
$2.08
10 µl
$3.05
3 µl
$0.96
6-well
2 ml
6 µl
$2.08
10 µl
$3.05
3 µl
$0.96
12-well
1 ml
3 µl
$1.04
4 µl
$1.22
1.5 µl
$0.42
 
 
 
 
 
 
 
 
 
 
 

  

 

Transfection efficiency comparison
 
Cells
BioT compares with
Lipofectamine-2000 and FuGENE
Cells
BioT compares with
Lipofectamine-2000 and FuGENE
 
HeLa
Better than both
ROS17-2
Similar to Lipo-2000, FuGENE
NIH3T3
Better than both
SaOS2
Similar to Lipo-2000, FuGENE
C2C12
Better than both
ST-2
Similar to Lipo-2000, FuGENE
MCF-7
Better than both
10T1/2
Similar to Lipo-2000, FuGENE
MNTT-1
Better than both
T47D
Similar to Lipo-2000, FuGENE
N2A
Better than both
TC-32
Similar to Lipo-2000, FuGENE
NCCIT
Better than both
MCF10A
FuGENE is better
PC12
Better than both
Human fibroblasts
FuGENE is better
RAW264.7
Better than both
Endothelial cells
FuGENE is better
COS
Similar to Lipo-2000, better than FuGENE
Primary Culture
HepG2
Similar to Lipo-2000, better than FuGENE
Mouse ES cells
Better than both
Hu-7
Similar to Lipo-2000, better than FuGENE
MEF
Better than both
HEK293
Similar to Lipo-2000, FuGENE
Mouse astrocytes
Better than both
CHO
Similar to Lipo-2000, FuGENE
Mouse mesenchymal stem cells
Better than both
RKO
Similar to Lipo-2000, FuGENE
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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