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PCR Cleaning kits

Introduction:

EasyPrepTM PCR Cleaning kit provide a fast and reliable method to purify DNA fragment from PCR, RFLP, phosphorylation, labeling and any other enzymatic reactions. DNA binds to the membrane in the presence of high concentration of salt. The other components such as nucleotides, enzymes, salts and dyes pass through the membrane and further washed away by Washing Buffer. Pure DNA is eluted with Elution Buffer (Tris buffer, 10 mM, pH8.5) or H2O. DNA fragments from 200 bp to 20 kb can be purified using the DNA mini binding column with up to 90% recovery rate for PCR cleaning.

 
Kit Contents:
Catalog No.
DC02-01
DC02-02
DNA mini Columns
50
250
Buffer PB*
25 ml
125 ml
DNA Wash Buffer (Concentrate)
12 ml
54 ml
Column Buffer
6 ml
30 ml
Elution Buffer
10 ml
20 ml
2ml Collection Tubes
50
250
User Manual
1
1
*Buffer PB contains chaotropic salts. Wear gloves and use protective eyewear when handling this solution. Buffer PB may form precipitates under cool ambient condition. Warm up the buffer at 37oC to dissolve before use.
 
Protocol:

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Product Image Catalog# Product Description Price
Buffer Set for Gel Extraction (250 preps) BF-DC01

Buffer Set for Gel Extraction (250 preps)

For 250 preps: 130 ml Buffer GB, 50 ml DNA Wash Buffer concentrate (for 250 ml 1x) and 20 ml Elution Buffer.  
$80.00     Add:

Buffer Set for PCR Cleaning (250 preps) BF-DC02

Buffer Set for PCR Cleaning (250 preps)

For 250 preps: 125 ml Buffer PB, 50 ml DNA Wash Buffer concentrate (for 1x250 ml) and 20 ml Elution Buffer.  
$80.00     Add:

Buffer GB (250 ml) BF06-01

Buffer GB (250 ml)

 For Gel Extraction kit (DC3511-01, DC3511-02)
$38.00     Add:

Buffer PB (250 ml) BF06-02

Buffer PB (250 ml)

 For PCR Cleaning kit 
$30.00     Add:

Columns for Gel extraction/PCR Cleaning (50 sets) COL03-01

Columns for Gel extraction/PCR Cleaning (50 sets)

Used for Gel extraction and PCR cleaning.  Column binding capacity: 15 ug Can elute with 15ul elution buffer Column Buffer included. Column Buffer is used for column equilibration, providing more consistent yield when the columns are stored for a while.  Compatible with all other...
$25.00     Add:

Columns for Gel extraction/PCR Cleaning (250 sets) COL03-02

Columns for Gel extraction/PCR Cleaning (250 sets)

Used for Gel extraction and PCR cleaning.  Column binding capacity: 15 ug Can elute with 15ul elution buffer Column Buffer included. Column Buffer is used for column equilibration, providing more consistent yield when the columns are stored for a while.  Compatible with all other...
$95.00     Add:

Gel extraction kit (50 Preps) DC01-01

Gel extraction kit (50 Preps)

  Extracting and purification of DNA fragment from agarose gels or enzymatic reactions (such as PCR).
$50.00     Add:

Gel extraction kit (250 Preps) DC01-02

Gel extraction kit (250 Preps)

  Extracting and purification of DNA fragment from agarose gels or enzymatic reactions (such as PCR).
$220.00  $198.00
Save: 10% off
     Add:

PCR cleaning kit (50 Preps) DC02-01

PCR cleaning kit (50 Preps)

  Purify DNA from enzymatic reactions (such as PCR) or chromatin immunoprecipitation (ChIP). Can not be used for gel extraction. Elution volume 35-50 ul.  
$50.00     Add:

PCR cleaning kit (250 Preps) DC02-02

PCR cleaning kit (250 Preps)

  Purify DNA from enzymatic reactions (such as PCR) or chromatin immunoprecipitation (ChIP). Can not be used for gel extraction. Elution volume 35-50 ul.
$198.00     Add:


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